ERa基因多态性与月经过少中医证候的相关性

Relation Between Estrogen Receptor-α Gene Polymorphisms and Different Traditional Chinese Medicine Syndromes of Hypomenorrhea

目的:探讨雌激素受体a(estrogen receptor-a,ERa)基因1号内含子Pvu II,Xba I基因多态性在月经过少不同中医证候间的分布情况。方法:采用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)分析方法检测月经过少患者外周血ERa基因多态性,比较不同中医证候间的Pvu II,Xba I基因型、等位基因计数的分布差异。结果:1 ERa Pvu II基因型PP,Pp,pp及Xba I基因型XX,Xx,xx在月经过少不同中医证候间的分布经Fisher确切概率法检验均有统计学差异(P<0.001)。其中pp基因型在的构成比依次为:肾虚(72.5%),血虚(7.14%),血瘀(6.25%);xx基因型的构成比依次为:肾虚(78.75%),血虚(28.57%),血瘀(6.25%)。2P,p和X,x等位基因频率在不同中医证候间的频率分布经χ2检验差异均有显著性(P<0.001)。p等位基因构成比:肾虚(83.75%),血虚(50%),血瘀(50%);x等位基因构成比:肾虚(88.75%),血虚(64.29%),血瘀(50%)。结论:在月经过少患者中,ERa基因多态性与月经过少中医证候存在相关性,p和x等位基因可能是肾虚证型月经过少的危险因素;pp和xx基因型是肾虚证月经过少的易感基因多态性。

Objective： This study was undertaken to find out the polymorphism variations in the estrogen receptor-a gene PvulI （rs2234693） and XbaI （rs9340799） in the different traditional Chinese medicine （TCM） syndromes of hypomenorrhea. Method： This case-control study used the polymerase chain reaction and restriction fragment length polymorphism （PCR-RFLP） analysis to find out the results. Result： ①The differences of genotype frequencies of ESR1 rs2234693 PP/Pp/pp genotype carriers were statistically significant among the different TCM syndromes of hypomenorrhea. The ESR1 rs9340799 XX/Xx/xx genotype carriers were statistically significant too （P 〈 0. 001 ）. The study showed that the genotype frequencies of ESR1 pp and xx genotype were higher in the kidney deficiency than other TCM syndromes of hypomenorrhea. ②The differences of allele frequencies of ESR1 P/p and X/x genotype carriers were statistically significant （P 〈 0. 001 ） among the different TCM syndromes of hypomenorrhea. The study showed that the genotype frequencies of ESR1 p and x alleles werehigher in the kidney deficiency than other TCM syndromes of hypomenorrhea. Conclusion： The results of the present study suggested that the ESR1 rs2234693 and rs9340799 gene were associated with the different TCM syndromes of hypomenorrhea. Patients with high frequencies of the p allele and x allele were more likely to respond to the hypomenorrhea with the kidney deficiency syndrome. The study showed that the ESR1 pp genotype and xx genotype were more susceptible to the kidney deficiency syndrome of hypomenorrhea.