基于代谢组学的中药配伍减毒研究策略探讨

Discussions on Research Strategies of Compatibility for Attenuating Toxicity of Herbs Based on Metabonomics

探讨应用代谢组学技术研究中药配伍减毒的策略。通过分析代谢组学技术应用于中药复方配伍减毒研究的优势,提出基于代谢组学的有毒中药配伍减毒研究方法和内容。代谢组学是继基因组学、转录组学和蛋白质组学之后发展起来的一门全新学科,具有整体、动态、综合与分析的特点。作为系统生物学的重要组成部分,代谢组学在中药配伍减毒研究中表现出很大的应用潜力。在中药复方配伍减毒研究中应用代谢组学的方法,应从基于药物代谢物组的毒性成分以及有效成分辨识、基于代谢组学的中药毒性靶器官以及毒性生物标志物的确定、基于代谢组学的对证控毒作用机制探究、基于系统建模的毒性成分组合与配伍减毒活性之间关联性分析4个方面进行。代谢组学应用于复方配伍减毒研究具有广阔的前景。

The aim of this study was to explore the application of metabonomics technology in the research on the attenuation strategy by compatibility of traditional Chinese medicine （TCM）. By analyzing the advantages of metabonomics used in the research on compatibility for attenuating toxicity of TCM, methods and content of compatibility for attenuating toxicity of TCM based on metabonomics was put forword. Metabolomics was a rapidly developed new technology after genomics, transcriptomics and proteomics, which is characterized by global, dynamic, comprehensive. Metabonomics studies display great potential in compatibility for attenuating toxicity of herbs as significant component of system biology. The application of metabonomics technology in the research on the attenuation strategy by compatibility of TCM should focus on four aspects, including identification of toxic components of drug metabolite group and effective ingredient, ascertainment of toxicity target organ of TCM and toxicity biomarkers, mechanism of toxicity control, and the analysis of correlation between toxic components of system modeling and attenuated activity of compatibility for attenuation synergia. Metabonomics has a vast potential in the research of attenuation strategy by TCM.