微流控芯片中磁珠表面免疫反应动力学研究

Kinetics of Immunoreaction on Magnetic Bead Surface in Microfluidic Chips

微流控芯片中磁珠作为固相载体的免疫分析得到了非常广泛的应用,了解磁珠表面动力学行为对于磁免疫分析技术的改进与提高具有重要意义。本文建立了一种实时监测微流控芯片中磁珠表面免疫反应的方法,探究了微流控芯片中流速和分析物浓度对磁珠表面偶联的小鼠IgG和溶液中Cy3标记的羊抗小鼠IgG反应动力学的影响,并获得相应的结合和解离速率常数,分别为7.9×104 L·mol-1·s-1,和1.7×10-4 s-1。

The bead-based immunoaffinity assay in microfluidic chips is currently an area of growing interest and good knowledge of binding kinetics on bead surface is critical for effective implementation of immunoassay technology.In this work,a method has been developed to monitor the reaction kinetics of antigen-antibody binding in magnetic bead-based microchip immunoassays in real-time.The influence of fluid velocity and antibody concentration on immunoreaction kinetics on magnetic bead surface was explored.By the above method,the association rate constant and dissociation rate constant of 7.9×10^4L·mol^-1·s^-1 and 1.7×10^-4s^-1 were obtained.