摘要
制备了多壁碳纳米管和壳聚糖纳米复合材料(Multi-walled Carbon Nanotubes and Chitosan Nano-composites,MWCNTs-CHIN)修饰丝网印刷电极(MWCNTsCHIN/SPE),分别采用循环伏安法(CV)和方波伏安法(SWV)考察了5-HT在修饰丝网印刷电极上的电化学特性。在优化条件下,应用SWV法,5-羟色胺在0.30V处可产生灵敏的响应电流,且与其浓度呈线性,线性范围为0.01-1.00μmol/L,线性相关系数R为0.9969,检出限(S/N=3)为0.005μmol/L。用该方法测定慢性不可预见性应激抑郁模型(Chronic Unpredictable Mild Stress,CUMS)小鼠大脑和血液中5-HT的含量,结果表明,氟西汀组与空白组中5-HT的含量相当,模型组中5-HT的含量明显低于空白组(P〈0.01)。
A novel disposable screen printed electrode(SPE)modified with the multi-walled carbon nanotubes and chitosan nano-composites(MWNTs-CHIN)for rapid detection of 5-hydroxytryptamine(5-HT)was established.A sensitive response to 5-HT was obtained on the MWNTs-CHIN/SPE at the potential of 0.30 V.Under the optimized conditions,square wave voltammetric(SWV)response of 5-HT showed that the oxidation peak current of 5-HT at MWNTs-CHIN/SPE was linear with its concentration in the range of 0.01-1.00μmol/L.The linear regression equation was I(μA)=3.7699c(μmol/L)+0.2319(R=0.9969),and the detection limit(S/N=3)was 0.005μmol/L.Murine models were established by chronic unpredictable mild stress(CUMS)and murine brain and blood samples were determinated.It showed that the content of 5-HT in blank group was comparable with that in fluoxetine group while dramatically higher than that in model group(P〈0.01).
出处
《分析科学学报》
CAS
CSCD
北大核心
2015年第2期159-164,共6页
Journal of Analytical Science
基金
国家中小企业创新基金(No.09C2621421479)
湖北省教育厅基金(No.Q20142806
XD2014282)
关键词
多壁碳纳米管
壳聚糖
丝网印刷电极
5-羟色胺
慢性不可预见性应激抑郁模型
Multi-walled carbon nanotubes
Chitosan
Screen printed electrode(SPE)
5-Hydroxytryptamine(5-HT)
Chronic unpredictable mild stress(CUMS)