硼酸亲和杂化毛细管整体柱选择性富集蒲公英中的活性物质

Specific Capture of cis-Diols Active Components in Taraxacum Mongolicum Hand.-Mazz.by a Hybrid Boronate Affinity Capillary Monolithic Column

以四乙氧基硅烷,N-氨乙基-3-氨丙基三乙氧基硅烷,3-甲基丙烯酰氧丙基三甲氧基硅烷作为前驱,4-乙烯基苯硼酸为单体,在毛细管中原位制备硼酸亲和杂化整体柱,该柱可在中性条件下选择性的富集顺式二羟基生物分子,实现了对蒲公英中活性成分咖啡酸及绿原酸的选择性富集。

A hybrid boronate affinity monolith was synthesized by using tetraethoxysilane（TEOS）,N-（β-aminoethyl）-γ-aminopropyltriethoxysilane（AEAPTES）,and γ-methacryloxypropyltrimethoxysilane（γ-MAPS）as the co-precursors and 4-vinylphenylboronic acid（VPBA）as the functionalized monomer.The prepared monolith can capture cis-diols active components,chlorogenic acid and cafeic acid,in Taraxacum mongolicum Hand.-Mazz.under neural conditions.