高效液相色谱法同时测定葛花中染料木苷和鸢尾苷的含量

Simultaneous determination of genistin and tectorigenin in Flos Puerariae by RP-HPLC

目的：建立葛花中染料木苷和鸢尾苷两个异黄酮成分的HPLC含量测定方法。方法：Agilent ZORBAX Eclipse XDBC18色谱柱（4.6 mm×150 mm,5μm）;以不同浓度的乙腈-1‰磷酸水为流动相梯度洗脱,流速0.8 ml·min-1,检测波长270nm,柱温30℃。结果：染料木苷和鸢尾苷的线性范围分别为46.7~747.2μg·ml-1（r=0.999 9）和54.1~864.8μg·ml-1（r=0.999 9）;平均回收率分别为101.1%（RSD为1.46%）和100.2%（RSD为1.58%）。结论：本方法简单,专属性强,重复性好,可用于葛花药材的质量控制。

OBJECTIVE To develop a HPLC method for determination of genistin and tectorigenin in Flos Puerariae.METHODS The samples were separated on a Agilent ZORBAX Eclipse XDB-C18column（4.6 mm×150 mm,5μm）with mobile phase consisting of acetonitrile-1‰ phosphoric acid solution（gradient elute）at the flow rate of 0.8 ml·min-1.Column temperature was set at 30℃ and wavelength was at 270 nm.RESULTS The linear ranges of the two components were 46.7~747.2μg·ml-1（r=0.999 9）and 54.1~864.8μg·ml-1（r=0.999 9）,respectively.The average recoveries of the two components were 101.1%（RSD=1.46%）and 100.2%（RSD=1.58%）,respectively.CONCLUSION The method is stable,accurate and simple,which can be used for quality control of Flos Puerariae.