野鸦椿籽中三萜类化合物的分离与鉴定

Isolation and identification of triterpenoids from fruits of Euscaphis fukienensis

目的对野鸦椿籽70%乙醇提取物的二氯甲烷及正丁醇萃取部位所含三萜类成分进行研究。方法采用硅胶、Sephadex LH-20等柱色谱方法进行分离、纯化,根据理化性质和波谱数据鉴定结构。结果从二氯甲烷及正丁醇部分分离得到8个单体化合物,分别鉴定为木鳖子酸(1)、熊果酸(2)、3β,19α-二羟基-乌索-12-烯-28α-酸(3)、β-谷甾醇(4)、胡萝卜苷(5)、白桦脂酸(6)、马斯里酸(7)、坡模酸(8)。结论三萜化合物1、2、3、6、7、8均是首次从该植物中分离得到。

AIM To investigate triterpennoids from methylene dichloride and n-butanol parts from 70% ethanol extract of the fruits of Euscaphis fukienensis. METHODS The triterpenoids were isolated and purified by silica gel and Sephadex LH-20 column chromatographies. Their structures were identified by spectroscopic methods and physicochemical properties. RESULTS Eight compounds were isolated and identified as momordic acid（ 1） 、ursolic acid（ 2） 、3β,19α-dihydroxyurs-12-en-28α-oic acid（ 3） 、β-sitosterol（ 4） 、daucostrol（ 5） 、betulinic acid（ 6） 、maslinic acid（ 7） 、pomonic acid（ 8）. CONCLUSION Triterpennoids 1、2、3、6、7、8 are isolated from the fruits of Euscaphis fukienensis for the first time.