HPLC法同时测定毛果婆婆纳中3种环烯醚萜苷

Simultaneous determination of three iridoid glycosides from Veronica eriogyne H. Winkl by HPLC

目的建立HPLC同时定量测定采自西藏、四川阿坝毛果婆婆纳中梓醇、桃叶珊瑚苷、胡黄连苷Ⅱ的方法。方法采用外标法,Gemini C18色谱柱（110,4.60 mm×250 mm,5μm）,流动相为乙腈（A）-水（B）梯度洗脱（0-13 min,3%A;13-20 min,3%-15%A;20-50 min,15%A;50-60 min,15%-21%A;60-70 min,21%A;70-110 min,21%-100%A）,体积流量1.0 m L/min,柱温25℃,检测波长203 nm。结果梓醇、桃叶珊瑚苷、胡黄连苷Ⅱ的线性范围分别为0.101 5-5.076μg,0.093 2-4.66μg,0.043 6-2.18μg;平均加样回收率分别为98.9%（RSD 1.0%）,103.8%（RSD 0.7%）,98.8%（RSD 1.9%）。结论 10个产地婆婆纳中梓醇、桃叶珊瑚苷、胡黄连苷Ⅱ的量差异明显,高低差异近10倍之多。

AIM To develop a method for determining catalpol,aucubin and picroside Ⅱ from Veronica eriogyne H. Winkl collected from Tibet and Si＇chuan Province by HPLC. METHODS External standard method and the chromatographic Gemini C18 Column（ 110 ,4. 60 mm × 250 mm,5 μm） were adopted with mobile phase consisting of acetonitrile（ A）-water（ B） in a gradient elution manner. The detected wavelength was set up at 203 nm and column temperature was maintained at 25 ℃. RESULTS The linear ranges were 0. 101 5- 5. 076μg for catalpol,0. 093 2- 4. 66 μg for aucubin,and 0. 043 6- 2. 18 μg for picroside Ⅱ,respectively. The average recoveries of catalpol,aucubin and picroside Ⅱ were 98. 9%（ RSD 1. 0%）,103. 8%（ RSD 0. 7%） and98. 8%（ RSD 1. 9%）,respectively. CONCLUSION The differences of contents of three constituents from V.eriogyne H. Winkl collected from ten growing areas are large,almost tenfold.