摘要
The present study was designed to isolate the polyphenol constituents of cultured cells of Saussurea involucrata. The polyphenol type constituents were isolated using chromatography methods, and then characterized by spectral analysis. 1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl)-hydrazyl(DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid(ABTS) free radical scavenging were assayed using Vitamin C as the positive control. One new polyphenol 18, 1, 3-di-O-caffeoyl-5-O-(1-methoxyl-2-O-caffeoyl-4-maloyl)-quinic acid, together with 17 known compounds, was isolated and characterized. In conclusion, Compound 18 was a new caffeoyl maloyl quinic acid type polyphenol and showed desired vitro anti-oxidant activity. Compounds 1–5, 9, 10, 14, 15, and 17 were isolated from cultured cells of Saussurea involucrata for the first time.
The present study was designed to isolate the polyphenol constituents of cultured cells of Saussurea involucrata. The polyphenol type constituents were isolated using chromatography methods, and then characterized by spectral analysis. 1,1-Diphenyl- 2-picrylhydrazyl radical 2,2-Diphenyl-1-（2,4,6-trinitrophenyl）-hydrazyl （DPPH） and 2,2＇-azinobis-（3-ethylbenzothiazoline-6-sulfonic acid （ABTS） free radical scavenging were assayed using Vitamin C as the positive control. One new polyphenol 18, 1, 3-di-O-caffeoyl-5-O-（1-methoxyl-2-O-caffeoyl-4-maloyl）-quinic acid, together with 17 known compounds, was isolated and characterized. In conclusion, Compound 18 was a new caffeoyl maloyl quinic acid type polyphenol and showed desired vitro anti-oxidant activity. Compounds 1-5, 9, 10, 14, 15, and 17 were isolated from cultured cells ofSaussurea involucrata for the first time.
基金
financially supported by National Major New Drugs Innovation and Development Program(No.2011ZX09307-002-01)
China Postdoctoral Science Foundation(No.2012T60303)
the National High Technology Research and Development Program("863" Program) of China(No.SS2014AA 022201)
