TRPC1在TGF-β1诱导支气管上皮细胞间充质转化中的作用

Effects of TRPC1 on TGF-β1-induced epithelial-mesenchymal transition of human bronchial epithelial cells

目的:探究经典瞬时受体电位通道1(TRPC1)在转化生长因子β1(TGF-β1)诱导人支气管上皮细胞(16HBE)间充质转化(EMT)中的作用。方法:以16HBE细胞株为研究对象,免疫荧光、RT-PCR和Western blotting检测16HBE细胞EMT过程中TRPC1 mRNA和蛋白的表达;Western blotting检测TRPC1阻断剂和siRNA干扰对16HBE细胞EMT的影响。结果:(1)TGF-β1刺激后细胞形态明显改变,E-钙黏蛋白表达减少(P<0.01),而α-SMA蛋白表达增加(P<0.05)。(2)TRPC1广泛存在于16HBE细胞,且TGF-β1刺激后TRPC1 mRNA和蛋白的表达增加(P<0.05)。(3)与TGF-β1组相比,阻断剂和TGF-β1共同作用组或siRNA和TGF-β1共同作用组细胞形态改变受抑制,E-钙黏蛋白和α-SMA蛋白表达受抑制(P<0.05)。结论:TGF-β1诱导16HBE细胞发生EMT,其机制可能与其上调16HBE细胞TRPC1有关。

AIM：To investigate the role of canonical transient receptor potential channel 1 （ TRPC1 ） in the epithelial-mesenchymal transition （ EMT） of human bronchial epithelial （ HBE） cells induced by transforming growth fac-tor-β1 （TGF-β1）.METHODS：EMT of 16HBE cells induced by TGF-β1 were identified by microscopy, immunofluores-cence and Western blotting.Immunofluorescence, real-time PCR and Western blotting were applied to detect the mRNA and the protein expression of TRPC1 in the 16HBE cells.The influence of SKF96365 （a TRPC1 blocker） and siRNA-me-diated silencing of TRPC1 on the EMT of the 16HBE cells were detected by microscopy and Western blotting.RESULTS：Treatment with TGF-β1 induced significant morphological changes of the 16HBE cells.Exposure to TGF-β1 decreased the expression of E-cadherin protein （P〈0.01） and increased the expression of α-SMA protein （P〈0.05） in the 16HBE cells.Immunofluorescence observation indicated that TRPC1 expression in the 16HBE cells was positive.The expression of TRPC1 at mRNA and protein levels was significantly increased in the 16HBE cells after stimulation with TGF-β1 （ P〈0.05）.The morphological changes of the 16HBE cells induced by TGF-β1 were inhibited by SKF96365 and TRPC1 silen-cing compared with TGF-β1 group.The protein expression of E-cadherin andα-SMA induced by TGF-β1 were inhibited by SKF96365 and TRPC1 silencing compared with TGF-β1 group （P〈0.05）.CONCLUSION：TGF-β1 induces EMT with the mechanism of up-regulating TRPC1 in human bronchial epithelial cells.