牙髓卟啉单胞菌超声提取物对成骨细胞周期和凋亡的影响

The effects of sonicated extract of Porphyromonas endodontalis on the cell cycle and apoptosis of osteoblasts

目的:观察牙髓卟啉单胞菌超声提取物对成骨细胞周期和凋亡的影响。方法:用不同浓度牙髓卟啉单胞菌超声提取物(1、10、100μg/ml)作用成骨细胞,MTT法检测细胞增殖情况,流式细胞仪分析细胞周期分布和凋亡变化。结果:牙髓卟啉单胞菌超声提取物显著抑制成骨细胞的增殖。且在一定时间和浓度范围内具有浓度和时间依赖性。10μg/ml和100μg/ml提取物作用24 h时,处于G1期的成骨细胞明显增多;在作用48 h时,牙髓卟啉单胞菌提取物以浓度依赖方式促进成骨细胞凋亡。结论:牙髓卟啉单胞菌超声提取物可阻滞细胞于G1期,并诱导细胞凋亡,抑制成骨细胞增殖。

Objective：To observe the effects of sonicated extract of Porphyromonas endodontalis（P.endodontalis）on the cell cy-cle and apoptosis of human osteoblastic hFOB 1 .1 9 cells.Methods：hFOB 1 .1 9 cells were treated with sonicated extract of P.end-odontalis at 0 （the control）,1 ,1 0 and 1 00 μg/ml for 1 2,24 and 48 h respectively.MTT assay was used to examine cell prolifera-tion.The cell cycle distribution and apoptosis were examined by flow cytometry.Results：The sonicated exact of P.endodontalis in-hibited the proliferation of hFOB 1 .1 9 cells in a time-and dose-dependent manner.24 h treatment of 1 0 μg/ml and 1 00 μg/ml ex-act arrested the cell cycle of the cells in G1 phase,48 h treatment induced apoptosis in a dose-dependent manner.Conclusion：Sonicated exact of P.endodontalis may arrest hFOB 1 .1 9 cells in G1 phase and induce cell apoptosis,leading to inhibition of the cell proliferation.