冷热循环对载银软衬材料白色念珠菌附着的影响

The effect of thermocycling on nano-silver-incorporated silicone denture liner for Candida albicans adhesion

目的:比较载银软衬材料老化前后对白色念球菌的抗菌效果。方法:制作10 mm×10 mm×3 mm规格载银和非载银软衬材料试件各16个,各取8个冷热循环4 000次处理;用白色念珠菌液对各试件黏附实验。结果:非载银材料冷热循环后细菌附着增加(P<0.05);载银材料冷热循环后细菌黏附数量无显著变化(P>0.05)。冷热循环与否,载银材料表面细菌粘附均少于非载银材料(P<0.01)。结论:老化的软衬材料易附着白色念珠菌,老化的载银软衬材料仍具有抗菌作用。

Objective：To evaluate the effect of thermocycling on nano-silver-incorporated silicone denture liner for Candida albicans （CA）adhesion.Methods：Specimens（1 0 mm×1 0 mm ×3 mm）of silicone denture liner（control）and that incorporated with nano-sil-ver were prepared according to the manufacturer＇s direction.8 of the each kind of specimens were immersed in distilled water for 24 h at 37 ℃,and 8 were subjected to thermocycling for 4 000 cycles at temperatures ranging from （5 ±1 ）℃to （55±1 ）℃with a 60 s dwell time per temperature.In vitro CA adhesion assay was used to measure the levels of adherence to the specimens.Data were analysed sta-tistically.Results：Thermocycling increased CA adhesion to control specimens（P〈0.05）,and did no change the adhesion to nano-sil-ver incorporated specimens（P〉0.05 ）.The adhesion of CA to nano-silver incorporated specimens was fewer than that to the controls with or wihout themocycling（P〈0.01 ）.Conclusion：Nano-silver incorporation may inhibit CA adhesion to silicone denture liner.Ther-mal cycling has no influence on C.albicans adhesion to silicone denture liner incorporated nano-sliver.