氟伐他汀钠对舌鳞癌Tca8113细胞基质金属蛋白酶-9表达影响的研究

The effect of fluvastatin sodium on MMP-9 expression in tongue squamous carcinoma Tca8113 cells

目的:探讨氟伐他汀钠对舌鳞状细胞Tca8113细胞基质金属蛋白酶-9(MMP-9)表达的影响。方法:不同浓度氟伐他汀钠干预细胞24 h,用荧光免疫组织化学法、Western Blot检测细胞MMP-9蛋白表达,对数据进行统计学分析。结果:实验组细胞较对照组细胞中的MMP-9表达明显减少(P<0.05),各实验组间随着药物浓度增加蛋白表达递减。2种试验方法结果一致。结论:氟伐他汀钠可以抑制舌鳞状细胞癌细胞中MMP-9蛋白表达,且为浓度依赖性。

Objective： To investigate the effect of fluvastatin sodium（FS） on MMP-9 expression in tongue squamous carcinoma Tca8113 cells. Methods： Immunofluoreseenee method and Western blot were used to detect the level of MMP-9 expression in Tca8113 cells treated by different concentration of FS for 24 h, the data were statistically analyzed. Results： MMP-9 expression in FS treated cells was significantly reduced（ P 〈 0.05 ） compared with control cells. With FS concentration increase the protein expression decreased in a dose-dependant manner detected by the 2 mothods. Conclusion： Fluvastatin Sodium can dose-dependantly inhibit the expression level of MMP-9 in TcaSll3 cells.