复方大黄散对佐剂性关节炎大鼠的作用研究

Effects of compound rhubarb powder on adjuvant arthritis in rats

目的:观察复方大黄散对佐剂性关节炎大鼠的作用及机制探讨。方法:用完全弗氏佐剂诱导佐剂性关节炎大鼠模型,设正常组、模型对照组、扶他林组、复方大黄散组,每组各12只。跖围法测量大鼠足跖肿胀度,酶联免疫法检测血清肿瘤坏死因子-α(tumor necrosis factor alpha,TNF-α)及白介素-10(interleukin-10,IL-10)的水平,光镜下观察踝关节组织形态学改变,免疫组化法检测踝关节TNF-α及IL-10的表达。结果:治疗6周后,与模型组(致炎14 d后跖围=3.50±0.12;血清TNF-α=88.90±3.90,踝关节TNF-α=9.25±0.54;血清IL-10=33.68±1.97,踝关节IL-10=0.25±0.13)比较,各治疗组大鼠于致炎14 d后足跖肿胀明显减轻(扶他林组=3.32±0.10;复方大黄散组=3.35±0.07;F=491.02;P=0.00);血清及踝关节TNF-α(扶他林组:血清TNF-α=77.32±2.65,踝关节TNF-α=4.83±0.30;复方大黄散组:血清TNF-α=75.90±1.95,踝关节TNF-α=4.83±0.30)水平均明显下降(血清:F=1 115.61,P=0.00;踝关节:F=122.39,P=0.00),而IL-10(扶他林组:血清IL-10=47.04±1.58,踝关节IL-10=5.83±0.39;复方大黄散组:血清IL-10=46.06±2.03,踝关节IL-10=5.50±0.36)水平均明显上升(血清:F=1 511.81,P=0.00;踝关节:F=133.94,P=0.00),扶他林组及复方大黄散组两者之间足跖肿胀度、TNF-α及IL-10水平均无统计学差异(足跖肿胀度:F=491.02,P=0.43;血清:TNF-α:F=1 115.61,P=0.21,IL-10:F=1 511.81,P=0.31;踝关节:TNF-α:F=122.39,P=1.00;IL-10:F=133.94,P=0.50);(光镜下)各治疗组大鼠关节组织中炎症细胞浸润减少,血管翳生成减少,关节腔间隙增宽,关节软骨破坏减轻。结论:复方大黄散能明显缓解佐剂性关节炎大鼠关节炎症,作用与扶他林相当,其机制可能通过下调致炎因子(TNF-α)、上调抗炎因子(IL-10),从而达到消除关节肿胀、缓解关节炎症的目的。

Objective：To observe the effects of compound rhubarb powder on adjuvant arthritis（AA）rats. Methods：The experimental AA model was set up by intradermal injection of freund＇s complete adjuvant（FCA）. Normal group,control group,voltaren-treated group and compound rhubarb powder-treated group were established with twelve in each group.The swollen volume of rats＇ claw was calculated by plantar circumference method. The morphology of ankle joint was observed under light microscopy. The levels of tumor necrosis factor-α（TNF-α）and interleukin-10（IL-10）in synovial fluid and blood serum and ankle joint were separately measured by ELISA and immunohistochemistry. Results：After six weeks of treatment,compared with those of control group,the swollen volume of rats＇ claw was significantly reduced（voltaren-treated group=3.32±0.10;compound rhubarb powder-treated group=3.35±0.07;F=491.02;P=0.00）in treatment group;the expression of TNF-α in blood serum（voltaren-treated group=77.32±2.65;compound rhubarb powder-treated group=75.90±1.95）and ankle joint（voltaren-treated group=4.83±0.30;compound rhubarb powder-treated group=4.83±0.30）was decreased（blood serum：F=1 115.61,P=0.00;ankle joint：F=122.39,P=0.00）;but the expression of IL-10（voltarentreated group：blood serum =47.04 ±1.58,ankle joint =5.83 ±0.39;compound rhubarb powder-treated group：blood serum =46.06 ±2.03,ankle joint=5.50±0.36）was increased（blood serum：F=1 511.81,P=0.00;ankle joint：F=133.94,P=0.00）. There was no statistical difference between voltaren-treated group and compound rhubarb powder-treated group in the swollen volume of rats＇ claw and the expression of TNF-α and IL-10（the swollen volume of claw：F=491.02,P=0.43;synovial fluid and blood serum：TNF-α：F=1 115.61,P=0.21;IL-10：F=1511.81,P=0.31;ankle joint：TNF-α：F=122.39,P=1.00;IL-10：F=133.94,P=0.50）. The inflammatory cells and pannus were reduced and articular cartilage was damaged less in each treatment group under light microscopy. Conclusion：Compound rhubarb powders can obviously decrease joint inflammation of AA rats as voltaren.Its mechanism may be related with decreasing inflammatory factors（TNF-α）and increasing antiinflammatory factors（IL-10）so as to eliminate joint swelling and reduce joint inflammation.