摘要
目的:探讨上调miR-30c的表达对大肠癌(CRC)细胞生物学行为的影响及可能的机制。方法:将miR-30c模拟物转染CRC细胞株SW620,qRT-PCR方法检测转染后miR-30c的表达,CCK-8法检测细胞增殖情况,Annexin V-FITC/PI双染色流式细胞术检测细胞凋亡情况,Transwell侵袭实验检测细胞侵袭能力的改变,qRT-PCR和Western blot检测转染后SNAI2 mRNA和蛋白的表达。结果:转染miR-30c模拟物后,SW620细胞的miR-30c表达明显增高,细胞增殖减少(P<0.05),凋亡增加(P<0.05),侵袭能力显著降低(P<0.05)。SNAI2 mRNA及蛋白表达水平明显下调,差异均有统计学意义(P<0.05)。结论:SW620细胞miR-30c表达上调,可抑制细胞增殖和侵袭、诱导细胞凋亡,其机制可能与抑制SNAI2表达相关。
Objective:To explore the effect and mechanism of miR -30c upregulation in CRC cells biological be- havior. Methods: After transfection of miR - 30c mimics into SW620 cells, the expression of miR - 30c was evaluated by real- time quantitative reverse transcriptase- polymerase chain reaction (qRT- PCR), and cell proliferation, ap- optosis and invasion was determined by CCK -8 assay, flow cytometry and transwell assay, respectively. SNAI2 mRNA and protein was measured by qRT - PCR and Western blot analysis. Results. miR - 30c mimics transfection signifi- cantly increased miR -30c expression in SW620 cells, inhibited cells proliferation and invasion, increased cells apop- tosis (P 〈0.05). miR- 30c mimics significantly suppressed SNAI2 mRNA and protein level in CRC cells (P 〈 0.05 ). Conclusion: Overexpression of miR - 30c in SW620 cells may suppress cells proliferation and invasion, in- duce cells apoptosis, probably via downregulating its target gene SNAI2 expression.
出处
《现代肿瘤医学》
CAS
2015年第8期1057-1061,共5页
Journal of Modern Oncology