IL-31RA基因敲除小鼠纯合子模型的建立

Establishment of IL-31RA Gene Knockout Homozygous Mice Model

目的:建立IL-31RA基因敲除小鼠纯合子模型,为IL-31RA基因相关研究提供动物模型。方法:IL-31RA基因敲除小鼠严格按照SPF级要求的动物饲养标准进行饲养繁殖,采用聚合酶链式反应(PCR)法鉴定子代小鼠的基因型,RT-PCR法鉴定小鼠IL-31RA mRNA的表达,Western blot鉴定IL-31RA蛋白的表达,HE染色观察小鼠重要脏器的形态学变化。结果:PCR法成功检测出子代小鼠的3种基因型,纯合子基因敲除小鼠未检测出IL-31RA mRNA和IL-31RA蛋白的表达,IL-31RA基因敲除小鼠的重要脏器的形态学特征与野生型小鼠比较无明显变化;基因敲除小鼠可成功饲养繁殖,亦可获得较多的基因敲除纯合子小鼠。结论:成功构建了IL-31RA基因敲除小鼠纯合子模型。

Objective： To establish the IL-31 RA gene knockout homozygous mice model and lay the foundation for further study on IL-31 gene. Methods： IL-31 RA gene knockout mice were bred and reproduced according to the SPF class animal feeding standard. PCR was used to identify the genotype of the offspring,the expression of IL-31 RA mRNA was detected by RT-PCR,expression of IL-31 RA protein was detected by Western blot,and morphological changes of vital organs were observed by HE staining. Result： Three genotypes of the offspring of IL-31 RA gene knockout mice were successfully identified; expression of IL-31 RA mRNA and IL-31 RA protein was not detected in IL-31 RA gene knockout homozygous mice. Compared with the wild type mice,morphological characteristics of vital organs of had no significant changes in IL-31 RA gene knockout homozygous mice. IL-31 RA gene knockout mice could be dred and reproduced successfully. Conclusion： The IL-31 RA gene knockout homozygous mice model has been successfully established.