不同时程海洛因依赖大鼠胃窦CD4、CD8和IL-2表达

The Expression of CD4,CD8 and IL-2 in Gastric Antrum of Heroin-dependent Rats in Different Periods

目的:探讨海洛因依赖期间大鼠胃窦内CD4、CD8及白细胞介素-2(IL-2)的表达。方法:正常成年SD大鼠,随机分为正常对照组(NCG)、盐水对照组(SCG组)和不同时程海洛因依赖组(HDG组);采用免疫组织化学SABC法及图像分析法,观察SCG组和HDG组造模第10、17、24、31及38天时大鼠胃窦黏膜CD4、CD8和IL-2表达。结果:与NCG组及SCG组比较,HDG组大鼠胃窦CD4、IL-2阳性细胞数量减少,染色变浅,图像分析的细胞计数和平均灰度值差异也有统计学意义(P<0.05);HDG组大鼠胃窦CD8阳性细胞较NCG及SCG组细胞数量增多,染色变深,在海洛因依赖第24天变化最为明显,差异有统计学意义(P<0.05)。结论:在海洛因依赖期间,CD4、IL-2在胃窦表达减弱,CD8表达增强,提示海洛因依赖对大鼠胃黏膜免疫功能造成损伤。

Objective： To explore the expression of CD4,CD8 and interleukin-2（ IL-2） in gastric antrum of heroin-dependent rats. Methods： Adult SD rats were divided into normal control group（ NCG）,saline control group（ SCG） and heroin-dependent group（ HDG）. Immunohistochemical SABC method and imagine analysis technique were performed to detect the expression of CD4、CD8 and IL-2 in the 10 th,17th,24 th,31stday after establishing heroin-dependent model. Results： The number of CD4,IL-2 positive cells and intensity of immunohistochemical staining of CD4,IL-2 in HDG were decreased,compard with NCG and SCG. The results of imagine analysis showed that the difference of the number and the average gray degree of CD4,IL-2 were statistically significant（ P 0. 05）. Compared with NCG and SCG,the intensity of immunohistochemical staining and the number of CD8 positive cells in gastric antrum in HDG increased,the most obvious change appeared at 24 thday of heroin dependence. Conclusions： The expression of CD4,IL-2 in gastric antrum is distinctly reduced,that of CD8 enhance during heroin dependence,suggesting that the immune function of rat antrum is damaged by heroin.