兔心搏骤停后综合征气管插管模型的建立

Establishment of tracheal intubation model following post-cardiac arrest syndrome in rabbits

目的建立一种损伤较小的兔气管插管方式，提高心搏骤停后综合征模型质量。方法选择大耳白兔30只，按随机数字表法分为三组：麻醉后直接气管插管（A组）、麻醉后切开逆向气管插管（B组）、麻醉后经皮穿刺逆向气管插管（C组），每组10只。静脉注射氯化琥珀胆碱后，在呼气末夹闭气管插管。当达到心搏骤停标准后，维持5min，然后开始进行常规心肺复苏，观察复苏前后动脉血压变化、心搏骤停后综合征发生情况和存活时间。结果心搏骤停A、B、C组心肺复苏成功率分别为40％、60％、80％（P〈0．01）；心搏骤停后综合征成功率分别为60％、80％、100％；动物存活时间中位数（四分位数）分别为23．4h（11．6～35．8h）、62．7h（29．4～88．6h）、79．5h（40．9～118．2h）（P〈0．01）。结论兔心搏骤停后气管插管持续时间以经皮穿刺逆向气管插管为适宜，此模型心肺复苏成功率及复苏成功后生存率均较高，且模型稳定，可重复性好，可作为心搏骤停后综合征研究的动物模型。

Objective To establish a less-damage method for tracheal intubation so as to improve the quality of post-cardiac arrest syndrome. Methods Thirty rabbits were divided into 3 groups of 10 rabbits each according to the random number table： group A receiving direct endotracheal intubation after anesthesia, Group B separation of cervical tissue and retrograde tracheal intubation after anesthesia and Group C percutaneous retrograde tracheal intubation after anesthesia. After the intravenous injection of forskolin, cardiac arrest was induced by endotracheal tube clamping. After 5 minutes of untreated arrest, conventional cardiopulmonary resuscitation was initiated. Changes in arterial pressure, occurrence of post-resuscition syndrome and survival time were examined in all groups. Results Groups A, B and C showed 40%, 60% and 80% success rate in cardiopulmonary resuscitation （ P 〈 0.01 ） and animal survival time of 23.4 hours （ 11.6 - 35.8 hours）, 62.7 hours （ 29.4 - 88.6 hours） and 79.5 hours （40.9 - 118.2 hours） （ P 〈 0.01 ）. Conclusions Percutaneous retrograde tracheal intubation is suitable to increase cardiopulmonary resuscitation rate and survival rate in rabbits with post-cardiac arrest. The model has good stability and repeatability and can be used for study of post-cardiac arrest syndrome.