摘要
用超声波法提取红果参果实的多糖,并分别以乙醇提取,乙酸乙酯、sevage试剂等进行纯化,以苯酚-硫酸法测定多糖含量,以GC-MS测定其单糖组分。结果表明,红果参果实中多糖含量高达45.80%,其主要单糖组分及百分比为阿拉伯糖(5.00%)、木糖(9.65%)、甘露糖(11.55%)、果糖(30.20%)、半乳糖(33.85%)。
Polysaccharides(PS) from the fruits of Campnumoea lancifolia were extracted and purified by ethanol precipitation, ethyl acetate and sevage reagent with ultrasonic assistance, and the content was determined by the phenol-sulfuric acid method. The components of the polysaccharides were identified by gas chromatography-mass spectrometry(GC-MS). The results showed that the average content of polysaccharide in the fruits of C. lancifolia reached up to 45.80%, and main monosaccharide composition and relative contents were arabia sugar(5.00%), xylose(9.65%), mannose(11.55%), fructose(30.20%), galactose(33.85%).
出处
《亚热带植物科学》
2015年第1期13-17,共5页
Subtropical Plant Science
基金
湖南省教育厅重点项目(09A073)
关键词
红果参
多糖
超声提取
单糖组成
GC-MS
Campnumoea lancifolia
polysaccharide
ultrasonic extraction
monosaccharide composition
GC-MS