摘要
目的探讨Slug在口腔鳞癌中的表达及在上皮间质化中的作用。方法采用免疫组织化学SP法和原位杂交法检测42例口腔鳞癌及12例正常口腔组织中Slug、E-cad、Vimentin的表达,对比Slug在两种组织中表达的差异,分析Slug与E-cad和Vimentin之间的关系,探讨Slug的表达与口腔鳞癌临床分期和病理分级的关系及其在上皮间质化中的作用。结果 Slug在口腔鳞癌中的阳性表达率明显高于正常组织,差异具有统计学意义(P<0.01);Slug在口腔鳞癌组织中的表达与淋巴结转移、肿瘤病理分级有关(P<0.05),与口腔鳞癌发病年龄无关(P>0.05);Slug与E-cad表达负相关,与Vimentin表达正相关。结论 Slug在口腔鳞癌的发生、分化及转移中起重要作用,可能在EMT的发生中起诱导作用。
Objective To investigate the expression of Slug in oral squamous cell carcinoma and its influence in the process of epithelial mesenchymal transitions. Methods The expression of Slug, E-cad, Vimentin in oral squamous cell carcinoma was detected by SP immunohistochemistry and in situ hybridization method in 42 specimens of oral squamous cell carcinoma and 12 specimens of oral normaltissue. Results The positive rate of Slug expression in oral squamous cell carcinoma was significantly higher than that in normal tissue（P〈0.01） ; The expression of Slug related to lymph metastasis and pathological grading（P〈0. 05）, but not related to the age of patients（P〉 0.05） ; The correlation between Slug and E-cad was negative, but was not between Slug and Vimentin. Conclusion Slug expression might play an important role in the development, metastsis of oral squamous cell carcinoma; EMT might be induced by Slug significantly.
出处
《青岛医药卫生》
2015年第2期81-85,共5页
Qingdao Medical Journal
