乙肝免疫标志物与乙肝病毒核酸含量与肝功能的关系研究

Relationship of Hepatitis B Immune Markers and HBV-DNA Content with Liver Function

目的:研究乙肝免疫标记物(HBV-M)与乙肝病毒脱氧核苷酸(HBV-DNA)载量与肝功能的关系。方法:检测104例乙肝患者HBs Ag、HBe Ag、HBc Ab、HBs Ab、HBe Ab5种HBV-M;用PCR法检测HBV-DNA含量;同时检测天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、胆碱酯酶(CHE)等肝功能指标,对三者关系采用Spearman相关性分析。结果:HBs Ag、HBc Ab、HBe Ag阳性组HBV-DNA阳性率及载量均大于其余组(P<0.05);HBe Ag含量、ALT浓度与HBV-DNA载量均呈正相关(r=0.48,P<0.05)、(r=0.36,P<0.05);AST、CHE与HBV-DNA含量无明显相关性,但在无病毒载量与有病毒载量组之间AST比较有统计学意义(P<0.05)。结论:HBV-M、HBV-DNA与肝功能之间有一定的相关性,但HBe Ag阴转不代表病毒复制停止,HBV-DNA也不能完全反应肝损害程度,临床应加大重视。

Objective： To study the relationship of HBV-M and HBV-DNA content with liver function. Methods： Detected the HBsAg, HBeAg, HBcAb, HBsAb and HBeAb levels of 104 hepatitis B patients. PCR method was used to detect their HBV-DNA levels. Meanwhile, their aspartate aminotransferase（AST）, alanine aminotransferase enzymes（ALT）, cholinesterase（CHE） levels were detected, and their relations between each other were obtained by using Spearman correlation analysis. Results： The positive rate of HBV-DNA and its load volume was greater in HBsAg, HBcAb, HBeAg-positive group than in the other groups（P〈0.05）. The HBeAg levels and ALT concentration was positively correlated with HBV-DNA load （r=0.48, P〈0.05）, （r=0.36, P〈0.05）. AST and CHE showed no significant correlation with HBV-DNA load, but AST between no viral group and viral group showed statistical difference （P〈0.05）. Conclusion： There is a certain correlation between HBV-M, HBV-DNA and liver fimction. But HBeAg seroconversion does not mean to stop viral replication, and yet HBV-DNA can not completely reflect liver damage. So we should pay more attention in clinic.