rhTNFR:Fc对兔正畸微种植体周围炎模型炎症反应的抑制作用研究

Effect of rh TNFR:Fc on the Peri-implantitis in Rabbit Model

[目的]评价注射用重组人Ⅱ型TNF受体-抗体融合蛋白(tumour necrosis factor FC fusion protein,rh TNFR:Fc)(商品名:益赛普)在兔正畸微种植体周围炎模型的作用。[方法]选用新西兰兔12只,随机分为模型对照组和治疗组。分别在兔下颌无牙区植入正畸微种植体,采用钢丝结扎法建立实验性微种植体周围炎模型,结扎56d,治疗组给予rh TNFR:Fc,剂量1.1mg mg/(kg·d)1周,模型对照组给予等量生理盐水,两组在造模后第14、56、63d检测改良菌斑指数(m PLI)、改良龈沟出血指数(m BI)和种植体周探诊深度(PD);造模后63d取种植体周围牙龈行组织病理学检查,常规HE、免疫组化染色,观察炎性细胞浸润及TNF-α分布情况。[结果]造模后63d,治疗组m PLI、m BI、PD较模型对照组均明显下降(P<0.05)。组织病理学检查显示:造模后63d治疗组牙龈组织内炎症细胞浸润、TNF-α均较模型对照组减少(P<0.05)。[结论]rh TNFR:Fc可影响微种植体周围炎炎症反应过程。

[Objective] The aim of the study was to evaluate the effect of recombinant human tumour necrosis factor FC fusion protein（rhTNFR：Fc） on the non-surgical treatment peri-implantitis in a rabbit modeL[Methods] 12 male adult rabbits were randomly chosen and divided into control group and treatment group. Peri-implantitis of micro implant in rabbit model was induced by securing silk ligatures around the implants in mandibular toothless alveolar for 8 weeks. Treatment group received rhTNFR：Fc（1.1mg·kg^-1 daily for 1 week） and control group received saline. Modified plaque and calculus index（mPLI）,modified gingival blooding index（mBI）,probing depths（PD） were evaluated before and after the application of rhTNFR： Fc.The level of inflannnatory cell infiltration and tumor necrosis factor-u in gingival tissues were measured. [Result] Compared with control group, mPLI, mBI and PD of the treatment group were significantly decreased after application of rhTNFR：Fc（P〈0.05）, inflammatory cell infiltration and TNF-α in treatment group was significantly lower（P〈0.05）. [Conclusion] rhTNFR：Fc could influence the course of the inflammatory response in peri-implantitis and might contribute to the treatment of peri-implantitis.