摘要
目的:建立重组SIV-h PEDF注射液病毒颗粒数检测实时定量PCR检测方法。方法:在提取重组SIV-h PEDF注射液供试品基因组RNA后,采用实时定量PCR的方法,检测供试品中的病毒颗粒数。结果:经过3次实验,所得SIV-h PEDF RNA标准品标准曲线均线性良好,重组SIV-h PEDF注射液供试品中病毒颗粒数检测结果无显著性差异。结论:所建立的重组SIV-h PEDF注射液病毒颗粒数实时荧光定量PCR检测方法重复性好、灵敏、准确,可用于重组SIV-h PEDF注射液病毒颗粒数的检测。
Objective: A Real-Time PCR method was developed to quantitatively determine the virus particles in recombinant SIV-h PEGF injection. Methods: After extracting the genome RNA in recombinant SIV-h PEDF injection, Real-time PCR was used to quantitatively determine the virus particles in test sample. Results: After 3 parallel experiments, the SIV-h PEDF RNA standard had a good linear, and there was no significant difference in the detection results of virus particles in recombinant SIV-h PEGF injection.Conclusion: The established method is repeatable, sensitive and accurate, and can be applied in the determination of virus particles in recombinant SIV-h PEGF injection.
出处
《现代生物医学进展》
CAS
2015年第8期1401-1405,共5页
Progress in Modern Biomedicine
基金
863计划项目(2012AA020805)
国家科技重大专项项目(2012ZX09304010)