肾康注射液对链脲佐菌素诱导的大鼠DN的作用研究

The Effects of Shenkang Injection on STZ induced Diabetic Nephropathy Rat Models

目的:研究肾康注射液(SKI)对糖尿病肾病(DN)的作用。方法:采用大鼠腹腔注射链脲佐菌素65 mg/kg体重建立DN大鼠模型,SKI高、中、低分别腹腔注射SKI 10 m L/kg,5 m L/kg,2.5 m L/kg,2次/天;正常组和模型组分别给予生理盐水5 m L/kg,2次/天;8周后,观察、测量相应生化和病理等指标。结果:SKI可明显增强DN大鼠肾脏对血肌酐和血尿素氮的清除率,显著降低血液中总胆固醇、甘油三脂和低密度脂蛋白的含量和升高血液中高密度脂蛋白含量,显著升高血清中T-SOD和CAT的活力,降低血清中NO及MDA含量和降低血清中NOS的活力,显著改善糖尿病引起的肾组织损伤。结论:SKI对治疗DN的治疗作用是肯定的其主要表现在改善血脂代谢紊乱,增强机体抗氧化应激能力及增强肾脏对肌酐和尿素氮的清除能力进而改善肾脏损伤。

Objective： To investigate the effects of Shenkang Injection（SKI） on diabetic nephropathy（DN）. Methods： DN rat models were established by STZ 65 mg/kg, intraperitoneal injection, and then divided into five groups; SKIH, SKIM and SKIL were give SKI 10 m L/kg, 5 m L/kg, 2.5 m L/kg, 2 times/d, respectively; While control group and model group were given saline 5 ml/kg, 2 times/d.After 8 weeks administration, the related biochemical indices were detected. Result： SKI could significantly decrease the level of Scr and BUN; And it notably decreased the concentrations of T-CHO, TG, LDL and increased the level of HDL in serum; It can increase the activity of CAT and T-SOD, decrease the concentrations of NO and MDA and inhibit the activity of NOS. Conclusion： SKI can relieve the damage by high glucose in renal and has obvious therapeutic effect on DN.