不同繁殖模式下甘草药用成分含量积累速度差异研究

Differences between Different Propagation Modes in the Accumulation Rate of the Content of Medicinal Components in Glycyrrhiza Uralensis Fisch

目的:系统比较野生甘草无性繁殖的甘草根和人工甘草种子有性繁殖的甘草根中药用成分的积累速度差异情况,为进一步研究影响甘草药用成分积累的基因机制奠定理论基础。方法:本研究基于HPLC和UV等分析方法,比较野生甘草和人工甘草在甘草酸、甘草苷和甘草总黄酮以及其他类成分的含量差异。基于HPLC指纹图谱技术,全面分析不同繁殖模式下甘草根的化学信息和内在质量的差异情况。结果:除甘草素和异甘草素外,野生甘草不定根中其他三种药用成分和总黄酮的含量均高于人工甘草实生根,经SPSS分析,除异甘草素外,其他成分在两组之间均存在显著差异;根据HPLC指纹图谱,两种甘草药用成分之间差异显著,相似度为0.647。结论:野生无性繁殖的甘草根和人工有性繁殖的甘草根中主要药用成分含量之间存在显著差异,野生甘草具有快速积累药用成分的功能。本研究结果对于今后人工调控生产优质甘草药材,实现甘草资源的可持续利用具有重要的理论和实践意义。

Objective： To systematically compare the difference in the accumulation rate of the content of medicinal components between licorice roots formed by asexual reproduction of wild licorice and sexual reproduction of cultivated licorice. Accordingly, this could be the theoretical basis to further study genetic mechanisms influencing the accumulation of medicinal components. Methods： In this study the contents of Glycyrrhizin, Liquiritin and total flavonoids and other types of components in wild and cultivated roots were analyzed by HPLC and UV; And based on HPLC fingerprint technology, the differences in the chemical information and the quality of licorice roots under different propagation modes were comprehensively analyzed. Results： Except for the contents of liquiritigenin and isoliquiritigenin, the contents of other three medicinal components and total flavonoids in wild root formed by asexual reproduction are higher than those in cultivated root formed by sexual reproduction. And all the contents of medicinal components but for isoliquiritigenin were different significantly by SPSS analysis; According to HPLC fingerprint, there were significant differences between wild and cultivated roots, and there was a low similarity of 0.647. Conclusions： There are significant differences between licorice roots formed by asexual reproduction of wild licorice and sexual reproduction of cultivated licorice in the accumulation rates of the content of medicinal components. And wild licorice has the function to accumulate the contents of medicinal components rapidly. The results could be of important theoretical and practical significance to regulate the production of high quality artificial licorice herbs and realize the sustainable utilization of licorice resource in the future.