分子标志物在子宫内膜癌中的表达分析

Expressions of the Molecular Biomarkers in Endometrial Carcinoma

目的:探讨EGFR、HER-2、P53、ER、Ki-67、PR、VEGF、TopⅡα、PDGFR-β、PDGFR-α十种分子标志物在子宫内膜癌肿瘤细胞中的表达情况。方法:利用免疫组织化学的方法研究解放军总医院病理科2010年到2012年199例子宫内膜癌中EGFR、HER-2、P53、ER、Ki-67、PR、VEGF、TopⅡα、PDGFR-β、PDGFR-α十种分子标志物的表达,表达结果使用x2检验进行统计。结果:分子标志物EGFR、HER-2、PDGFR-α在子宫内膜癌肿瘤细胞中的表达与临床手术分期相关;ER、PR在子宫内膜癌肿瘤细胞中的表达与病理分级、临床手术分期及肌层浸润程度均相关。P53、Ki-67、VEGF、TopⅡα、PDGFR-β五个分子标记物与病理分级、临床手术分期及肌层浸润程度无明显相关性。结论:对十种分子标志物在子宫内膜癌肿瘤细胞中的表达分析,以及与病理分级、临床手术分级和肌层浸润程度关系的研究,有助于预测子宫内膜癌的发生、发展、转移及预后。

Objective： To investigate the expression of ten molecular biomarkers which are EGFR, HER-2, P53, ER, Ki-67, PR,VEGF, Top Ⅱ α, PDGFR-β and PDGFR-αin endometrial carcinoma. Methods： Immunohistochemical method was used to detect the expression of EGFR, HER-2, P53, ER, Ki-67, PR, VEGF, Top Ⅱ α, PDGFR- β and PDGFR-α in 199 cases of endometrial carcinoma which were from January 2010 to November 2012 in our department, then x2 test was used to analyze the data. Resultss： The positive expression rate in endometrial carcinoma of EGFR, HER-2 and PDGFR-α were correlated closely with clinical stage. And the positive expression rate in endometrial carcinoma of ER and PR were correlated closely with clinical stage, histological grade and myometrial invasion. P53, Ki-67, VEGF, TopⅡα and PDGFR-β were not correlated closely with clinical stage, histological grade and myometrial invasion. Conclusions： Detection of expression of ten molecular biomarkers in endometrial carcinoma may be helpful to predict the pathogenesis, progression, metastasis and prognosis of endometrial carcinoma.