苍术提取物对脾虚证大鼠胃粘膜及胃肠免疫功能的影响

Effect of Rhizoma Atractylodis extract in protecting gastric mucosa and modulating gastrointestinal immune function in a rat model of spleen deficiency

目的探讨苍术提取物(Rhizome Atractylodes extract,ERA)保护胃粘膜的作用及对脾虚模型大鼠胃肠免疫功能的影响,阐明ERA干预脾虚证的作用机制。方法采用喂饲小承气汤煎剂加饥饱失常建立大鼠脾虚证模型,模型复制成功后动物随机分为脾虚模型组,ERA高、中、低剂量组,多潘立酮组。连续灌胃给药10 d。采用肠道灌流法检测大鼠肠道灌流液Ig A含量,腹主动脉采血法检测大鼠血清Ig G含量,并测定大鼠胸腺、脾脏指数,HE染色行大鼠胃黏膜病理学观察,激光多普勒微循环血流计行大鼠胃黏膜血流量的测定,免疫组化法测定大鼠胃黏膜组织中TFF1及结肠TLR4的表达量。结果与正常组比较,模型组大鼠胃黏膜形态学、胃黏膜血流量、相关免疫学指标显著改变;与模型组比较,ERA各剂量组大鼠胃黏膜形态学、胃黏膜血流量、胃黏膜组织中三叶因子1(TFF1)的表达量,肠道灌流液Ig A、血清Ig G含量、胸腺、脾脏指数及结肠TLR4的表达量不同程度升高,差异均有统计学意义(P<0.05或P<0.01)。结论 ERA可抑制脾虚证大鼠胃粘膜损害,保护和修复损伤的粘膜组织,并改善脾虚证大鼠的免疫功能。

Objective To investigate the effect of Rhizoma Atractylodis extract（ERA） in protecting gastric mucosa and modulating gastrointestinal immune function of a rat model of spleen deficiency syndrome and elucidate the mechanism by which ERA improves spleen deficiency syndrome. Methods Male rats were fed with Xiaochengqi decoction and subjected to irregular feeding to induce spleen deficiency syndrome. The established models were randomized into model group, high-,moderate- and low-dose ERA groups, and domperidone group. After corresponding treatment for 30 days, the content of Ig A in the intestinal lavage fluid, serum Ig G, and the indices of the spleen and thymus were determined. The pathological changes in the gastric mucosa was observed with HE staining, gastric mucosal blood flow was evaluated with laser Doppler rheometry,and the expression of TFF1 in the gastric mucosa and TLR4 expression in the colon tissue were detected with immunohistochemistry. Results The rat models of spleen deficiency syndrome showed obvious abnormalities in gastric mucosal morphology, blood flow and immunological indexes. Compared with the model rats, the rats receiving ERA treatment as different doses all showed significant improvements in gastric mucosal morphology, blood flow volume, gastric mucosa trefoil factor 1（TFF1） expression, intestinal lavage fluid Ig A content, serum Ig G content, indices of the spleen and thymus, and TLR4 expression in the colon TLR4（P〈0.05 or P〈0.01）. Conclusion ERA can inhibit gastric mucosal damage,protect and repair the damaged mucosal tissues, and improve the immune function of in rats with spleen deficiency.