超顺磁性氧化铁纳米粒子增强Jurkat T细胞的活力和增殖能力

Superparamagnetic Iron Oxide Nanoparticles Enhance the Proliferation and Viability of Jurkat Cells

目的观察超顺磁性氧化铁纳米粒子(SPION)对T淋巴细胞型白血病细胞株Jurkat E6-1细胞增殖和活力的影响。方法常规培养Jurkat E6-1细胞,将一定浓度的SPION加入经CFSE负载的Jurkat细胞中不同时间,然后用PBS将细胞外的SPION洗去,再换用完全培养基培养一定时间,最后采用流式细胞术定量检测Jurkat细胞的增殖情况,用CCK-8细胞活力测定试剂盒检测Jurkat细胞的活力。结果 SPION(20μg/ml)促进Jurkat细胞增殖,使已分裂细胞百分比增高(与对照值相比,P<0.05),分裂指数增高(与对照组相比,P<0.05);SPION也使Jurkat细胞的活力显著增强(与对照组相比,P<0.01)。此外,SPION对Jurkat细胞有钙动员作用,可使Jurkat细胞的胞内游离钙水平显著增高。结论 SPION能促进Jurkat细胞增殖,并增强其活力;这些效应与其钙动员作用有关。

Objective To investigate the effects of superparamagnetic iron oxide nanopartieles （SPION） on the proliferation and via- bility of Jurkat cells. Methods Jurkat E6 - 1 cells were conventionally cultured. SPION （20μg/ml） were added to CFSE - loaded Jur- kat cells to act for different times, cells were then washed with PBS and were cultured for a period of time. Then, flow cytometry was used to quantitatively measure the proliferation, and CCK -8 method to evaluate the viability, of Jurkat E6 - 1 cells. Results SPION （20μg/ ml） enhanced the proliferation of Jurkat cells, as shown by the increased percentage of devided Jurkat cells compared with that of the con- trol （ P 〈 0. 05 ） and by the increased division index （DI） of Jurkat ceils （ P 〈 0. 05 vs control）. SPION also increased the viability of Jur- kat cells （P 〈 0.01 vs control）. In addition, SPION exerted a calcium mobilizing effect on Jurkat cells, and it significantly increased the intracellular free Ca2 ＋ level of Jurkat cells. Conclusion SPION enhances the proliferation and viability of Jurkat cells, and these effects are likely associated with its calcium mobilizing effect.