GRP78和Mn-SOD表达与鼻咽癌组织放疗敏感相关性研究

Relative Expressions of GRP78 and Mn-SOD in Radiotherapy of Nasopharyngeal Carcinoma

目的 研究GRP78、Mn-SOD mRNA在放射治疗鼻咽癌中的表达,探讨其在放射治疗鼻咽癌中的意义.方法 取60例2006年1月13日～11月26日第1次入院放射治疗鼻咽癌,运用原位杂交方法检测放射治疗鼻咽癌中GRP78及Mn-SODmRNA的表达.结果 在放疗敏感及放疗抗拒鼻咽癌患者中,GRP78 mRNA中度和强阳性表达率分别为20.00％和47.50％,Mn-SOD mRNA中度和强阳性表达率分别为55.00％和40.00％,差异有统计学意义.在放疗抗拒鼻咽癌患者中,GRP78 mRNA中度和强阳性表达率与TNM分期呈正相关,Ⅲ～Ⅳ期GRP78 mRNA中度和强阳性表达率为58.62％,Ⅰ～Ⅱ期表达率为18.18％,与T分期呈正相关,T3～T4期中表达率为68.42％,T1～T2期中表达率为28.57％;Mn-SOD mRNA中度和强阳性表达率与T分期呈正相关,T3～T4期中表达率为63.16％,T1～T2期中表达率为19.05％;在有、无远处转移中,GRP78 mRNA中度和强阳性表达率分别为72.73％和37.93％,Mn-SOD mRNA中度和强阳性表达率分别为81.82％和24.14％,差异有统计学意义（P＜0.05）.结论 GRP78、Mn-SOD参与了放疗抗拒鼻咽癌的发展.

Objective To study the expressions of GRP78 and Mn - SOD in the radiotherapy of nasopharyngeal carcinoma, as well as their slgnificances. Methods Radiotheraples were taken in 60 cases of nasopharyngeal carcinoma from 2006.1.13 - 2006.11.26 in situ hybridization detections were carried out to detect the expression of GRP78 and Mn - SOD mRNA. Results In radiosensitivity and radioresistance of nasopharyngeal carcinoma, the moderately and strongly positive GRP78 mRNA expression rates were 20.00% and 47.50% , the moderately and strongly positive Mn - SOD mRNA expression rates were 55.00% and 40.00% , and the differences were statistical significance. In the radioresistance of nasopharyngeal carcinoma, GRP78 mRNA moderately and strongly positive expression was positively correlated with TNM stage. In the degree of llI- IV, it had moderately and strongly positive expression rate of 58.62%. In the degree of I - lI, it had moderately and strongly positive expression rate of 18.18% ,and it was also positively correlated with T stage. In the degree of T3 - T4 , it had moderately and strongly positive expression rate of 68.42%. In the degree of T1 - T2 , it had moderately and strongly positive expression rate of 28.57%. Mn - SOD mRNA moderately and strongly positive expression was positively correlated with T stage. In the degree of T3 - T4 , it had moderately and strongly positive expression rate of 63.16%. In the degree of Tl - T2 , it had moder- ately and strongly positive expression rate of 19.05%. In distant metastasis and no distant metastasis, the moderately and strongly positive GRP78 mRNA expression rates were 72.73% and 37.93% , the moderately and strongly positive Mn - SOD mRNA expression rates were 81.82% and 24.14% , and the differences had statistical significance. Conclusion GRP78 and Mn - SOD have participated in the de- velopment of radioresistant nasopharyngeal carcinoma.