鼻息肉组织VEGF、TGF-β1表达水平与病理特征及微血管密度的相关性

Correlation between Expression of VEGF,TGF-β1 in Tissue of Nasal Polyps and Pathological Characteristics and MVD

目的探讨鼻息肉组织血管内皮细胞生长因子(VEGF)、转化生长因子-β1(TGF-β1)表达水平及其与病理特征及微血管密度(MVD)的相关性。方法应用免疫组织化学法检测68例鼻息肉组织及30例下鼻甲黏膜组织中VEGF、TGF-β1及CD34的表达水平,分析其与病理特征的关系。结果鼻息肉组织中VEGF、TGF-β1蛋白的阳性表达率分别为58.82%、66.18%,显著高于下鼻甲黏膜组织中的阳性表达率16.67%、20.00%(χ2=14.90、17.78,P<0.05),且鼻息肉组织中VEGF、TGF-β1阳性率与病理类型、临床分期、息肉数、复发与否明显相关(P<0.05);鼻息肉组织中的MVD计数明显高于下鼻甲黏膜组织(P<0.05),Ⅱ型3期、Ⅲ型患者MVD显著高于Ⅱ型1期、2期,嗜酸粒细胞性、复发、多发者MVD高于嗜中性粒细胞性、非复发、单发者(P<0.05);鼻息肉组织VEGF、TGF-β1表达水平与MVD计数水平呈正相关(r=0.641、0.778,P<0.05)。结论鼻息肉组织中VEGF、TGF-β1呈过度表达状态,且与MVD计数呈正相关,共同参与了鼻息肉发生、发展的过程,有望为鼻息肉的治疗提供新的思路。

Objective To study the expression of vascular endothelial growth factor （VEGF） , transforming growth factor - β1 （TGF- β1） in tissue of nasal polyps, and explore its correlation with pathological characteristics and microvaseular density （MVD）. Methods The expression of VEGF, TGF - β1 and CD34 in the tissue of nasal polyps （n = 68） and inferior turbinate mucosa （ n = 30） were detected by using immunohistoehemieal staining. The correlation between three indicators above and pathological features were ana- lyzed. Results The positive expression rate of VEGF, TGF - β1 protein in nasal polyps was 58.82% , 66.18% , which was significantly higher than 16.67% ,20.00% of inferior turbinate mucosa （X2 = 14.90, 17.78, P 〈 0.05 ）. The rates were remarkably correlated with pathological type, clinical stage, number of polyps, recurrence （P 〈 0.05）. There was significant difference on the count of MVD in na- sal polyps and inferior turbinate mucosa （P 〈 0. 05）. The expression of MVD in nasal polyps of type II phase 3 and type Ⅲ was higher than that in nasal polyps of type Ⅱ phase 1 and type Ⅱ phase 2, and the expression of MVD in eosinophilic, recurrent, multiple nasal polyps was higher than that in neutrophil, non - recurrent, single nasal polyps（P 〈 0.05）. The count of MVD in breast cancer was posi- tive correlated with VEGF, TGF - β1 （ r = 0. 641,0. 778, P 〈 0.05）. Conclusion The expression of VEGF, TGF - β in tissue of nasal polyps is over - expressive, and positive related with MVD. They are involved in the process of the development of nasal polyps, and would be expected to provide a new method for the treatment of nasal polyps.