摘要
Objective： TO study the prevention effect of salidroside on contrast-induced-nephropathy （CIN） and its underlying mechanism. Methods： A total of 24 Wistar rats were randomly divided into 4 groups with 6 in each group. Rats were firstly administrated with normal saline （control and model groups）, N-acetylcysteine （NAC, NAC group） and salidroside （salidroside group） for 7 days before model establishment in each group, respectively. Histopathological analysis was performed by periodic acid-Schiff （PAS） staining. Oxidative stress related parameters including superoxide dismutase （SOD） and methane dicarboxylic aldehyde （MDA）, nitric oxide （NO）, angiotensin 11 （Ang II）, 8-hydroxy-2＇-deoxyguanosine （8-OHdG）, mRNA and protein levels of endothelial nitric oxide synthase （eNOS）, and nitric oxide synthase （NOS） activity were measured. Results： Compared with the control group, the levels of MDA, Ang II and 8-OHdG were all significantly increased and levels of SOD, NO, and eNOS mRNA and protein were decreased significantly in the model group （P〈0.05）. Meanwhile, the NOS activity was also significantly decreased in the model group （P〈0.05）. In addition, the levels of these parameters were all improved in the NAC （P〈0.05） and salidroside groups and no significant different was found between these two groups （P〉0.05）. Conclusion： Salidroside can be the potential substitute Of NAC to prevent CIN. The underlying mechanism may be associated with oxidative stress damage caused by contrast agents.
Objective： TO study the prevention effect of salidroside on contrast-induced-nephropathy （CIN） and its underlying mechanism. Methods： A total of 24 Wistar rats were randomly divided into 4 groups with 6 in each group. Rats were firstly administrated with normal saline （control and model groups）, N-acetylcysteine （NAC, NAC group） and salidroside （salidroside group） for 7 days before model establishment in each group, respectively. Histopathological analysis was performed by periodic acid-Schiff （PAS） staining. Oxidative stress related parameters including superoxide dismutase （SOD） and methane dicarboxylic aldehyde （MDA）, nitric oxide （NO）, angiotensin 11 （Ang II）, 8-hydroxy-2＇-deoxyguanosine （8-OHdG）, mRNA and protein levels of endothelial nitric oxide synthase （eNOS）, and nitric oxide synthase （NOS） activity were measured. Results： Compared with the control group, the levels of MDA, Ang II and 8-OHdG were all significantly increased and levels of SOD, NO, and eNOS mRNA and protein were decreased significantly in the model group （P〈0.05）. Meanwhile, the NOS activity was also significantly decreased in the model group （P〈0.05）. In addition, the levels of these parameters were all improved in the NAC （P〈0.05） and salidroside groups and no significant different was found between these two groups （P〉0.05）. Conclusion： Salidroside can be the potential substitute Of NAC to prevent CIN. The underlying mechanism may be associated with oxidative stress damage caused by contrast agents.
基金
Supported by the National Nature Science Foundation of China(No.81273968 and No.81471027)
Ministerial Project of the National Working Commission on Aging(No.QLB2014W002)
