模拟微重力对人牙髓干细胞在裸鼠体内矿化能力的影响

Effect of Simulated Microgravity on Mieralization of Human Dental Pulp Stem Cells on PLGA Scaffolds in Vivo

目的:研究模拟微重力环境对聚乳酸-羟基乙酸(PLGA)支架上的人牙髓干细胞(human dental pulp stem cells,hDPSCs)在裸鼠体内矿化能力的影响。方法:分离、培养hDPSCs并进行鉴定,然后将hDPSCs接种到PLGA支架上培养72h后随机分为两组,普通重力组和模拟微重力组,经矿化诱导液培养1周后分别将细胞支架复合物移植到裸鼠体内,植入术后4周取材,进行组织学(HE和Vonkossa)和免疫组织化学(DSPP)检测。结果:HE染色均可见有血管生成,Vonkossa染色均为阳性,模拟微重力组DSPP的表达水平明显高于普通重力组(P<0.05)。结论:模拟微重力有利于hDPSCs在裸鼠体内的矿化。

Objective.. To investigate the mineralized effect of simulated microgravity on human dental pulp stem cells（hDPSCs） on PLGA（poly lactic--co--glycolic acid） after subcutaneous transplantation into nude mice. Meth- ods： hDPSCs were seeded into PLGA scaffolds and cultured for 72 hours, then cell--scaffold composites were ran- domly divided into 2 groups： normal gravity group and simulated microgravity group, which were both cultured in osteogenic induction medium. After 1 week, cell--scaffold composites of both groups were respectively transplanted into the subcutaneous tissue of immunodeficiency mice. The samples were retrieved after 4 weeks of implantation for histological（HE and Vonkossa） and immunohistochemical （DSPP） examinations. Results.. Angiogenesis was visualized in HE stained sections. Vonkossa staining were positive for assessing mineral deposition. The expression level of DSPP in simulated microgravity group was significant higher than that in normal gravity group（P〈.05）. Conclusion： Simulated microgravity is advantageous for the mineralization of hDPSCs in nude mice.