白杨素对大鼠脓毒症相关急性肺损伤的作用和可能机制

Effect and possible mechanisms of Chrysin on sepsis-associated acute lung injury of rats

目的应用白杨素对大鼠急性肺损伤(ALI)进行干预,探讨白杨素对脓毒症相关ALI的作用和可能机制。方法 72只Wistar大鼠随机分为正常对照组、LPS造模组、白杨素高剂量组(C-H,30mg/kg)和白杨素低剂量组(C-L,10mg/kg),每组18只大鼠。各组大鼠进行右肺上叶切除,行病理组织学检查;中叶切除,进行肺组织湿/干重(W/D)的重量比。采用免疫组化法、Western blot法和Real-time-PCR法检测肺组织HMGB1的蛋白和m RNA表达水平。结果与正常对照组相比,LPS诱导ALI组的W/D比值较高(<0.05),应用白杨素治疗后,W/D比值显著降低(<0.05)。与对照组相比,ALI组大鼠光镜下可见肺泡壁的增厚,肺水肿及肺泡塌陷,严重出血且有明显的炎性细胞浸润,经白杨素治疗后,炎性减轻。ALI组大鼠肺组织HMGB1蛋白和m RNA表达水平显著高于对照组(<0.05),而C-H和C-L组比ALI组明显减少(<0.05)。结论白杨素对ALI大鼠肺脏的保护作用可能与抑制炎症细胞因子HMGB1的表达相关。

Objective To investigate the effect and possible mechanism of chrysin on sepsis related acute lung injury（ALI） of Wistar rats. Methods 72 Wistar rats were assigned averagely into 4 groups： control group（NS）, ALI group, high doses of chrysin group（C-H,30mg/kg） and low doses of chrysin group（C-L,10mg/kg）. The superior lobe of the right lung was excised for HE examination. The middle lobe was excised for analysis of the lung wet/dry（W/D） weight ratio. Western blot, immunohistochemistry and Real-time-PCR were employed to analysis HMGB1 expression in lung tissue. Results The W/D Ratio was higher in LPS-induced ALI group than in control group, but reduced after chrysin treatment（ P〈0.05）. The widespread thickned alveolar wall and edema, alveolus collapse, severe hemorrhage in the alveolus and obvious inflammatory cells infiltration were found in LPS-induced ALI group but not in control group, however, chrysin treatment significantly attenuated the histopathological results in ALI group. The expression levels of HMGB1 protein and m RNA in lung tissue were higher in LPSinduced ALI group than in control group, but lower in C-H group and C-L group than in LPS-induced ALI group. Conclusion The protective effect of Chrysin on the lung tissues of ALI rats might be related to suppressing the expression of HMGB.