γ-促分泌酶抑制剂对哮喘小鼠肺组织IL-4表达的影响被引量：3

The effect of γ-secretase inhibitor on the expression of IL-4 in lung of asthmatic mouse

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摘要目的探讨γ-促分泌酶抑制剂对哮喘模型小鼠肺组织白介素-4表达的影响。方法选取30只BALB/c小鼠,随机分为对照组、哮喘组和γ-促分泌酶抑制剂阻断组。免疫组织化学方法和ELISA方法检测各组小鼠肺组织内IL-4的表达。结果哮喘组小鼠肺组织IL-4的表达水平显著高于正常对照组(<0.01),但是在应用γ-促分泌酶抑制剂处理后,哮喘组小鼠肺组织IL-4的表达水平显著下调(<0.01)。结论静脉注射γ-促分泌酶抑制剂能降低哮喘小鼠肺组织IL-4的表达。 Objective To study the effect of γ-secretase inhibitor on the expression of interleukin-4（IL-4） in lung of asthmatic mouse. Methods 30 BALB/c mice were randomly divided into normal control group, asthmatic group and γ-secretase inhibitor group. The expression of IL-4 was observed by immunohistochemistry and enzyme-linked immunosorbent assay（ELISA） methods. Results Compared with the normal control group, the expression level of IL-4 in lung of mice of the asthmatic group was increased significantly, but decreased significantly after γ-secretase inhibitor was injected by vena caudalis compared with the asthmatic group. Conclusion γ-secratase inhibitor could down-regulate the expression of interleukin-4 in lung of the asthmatic mouse.

作者席洪刚陈泽权

机构地区葫芦岛市中心医院检验科葫芦岛市妇幼保健院检验科

出处《解剖科学进展》 CAS 2015年第2期153-155,共3页 Progress of Anatomical Sciences

基金辽宁省博士启动基金(No.20111123)

关键词哮喘 NOTCH γ-促分泌酶抑制剂肺小鼠 asthma Notch γ-secretase inhibitor lung mice

分类号 R562.25 [医药卫生—呼吸系统]

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