抗感染对伴炎症兔颊VX2鳞状细胞癌组织中树突状细胞表面分子HLA-DR和CD86表达的影响

Effects of anti-infection treatment on expressions of HLA-DR and CD86 in dendritic cells in rabbit buccal VX2 squamous cell carcinoma tissue with inflammation

目的探讨抗感染对伴炎症兔颊VX2鳞状细胞癌(鳞癌)组织中树突状细胞(DCs)表面分子HLA-DR和CD86表达的影响。方法通过接种VX2瘤粒、形成机械创伤、饮用高糖黏性牛奶的方式,建立兔颊VX2鳞癌炎症模型。实验分为4组。A组(12只):颊癌伴炎症模型兔,连续3 d普鲁卡因青霉素肌注和替硝唑片灌胃。B组(12只):颊癌伴炎症模型兔,连续3 d生理盐水肌注和阿司匹林灌胃。C组(12只):颊癌伴炎症模型兔,伴有局部炎症的兔颊癌,连续3 d生理盐水肌注和灌胃。D组(10只):单纯颊癌模型兔,连续3 d生理盐水灌胃和肌注。实验结束后处死所有兔,收集肿瘤标本,流式细胞仪检测肿瘤组织中DCs表面分子HLA-DR和CD86的表达。结果 HLADR单独表达率、HLA-DR和CD86同时表达率均为A组>D组>B组>C组,CD86单独表达率为A组>D组>B组及C组(P<0.05)。结论抗感染治疗能明显提高伴炎症的兔颊癌组织中DCs表面分子HLA-DR和CD86的表达。

Objective To investigate the effects of anti-infection treatment on the expressions of antigen-presenting-related membrane-surface molecules HLA-DR and CD86 in dendritic cells （DCs） in rabbit buccal VX2 squamous cell carcinoma tissue complicated with local inflammation. Methods Rabbit buccal VX2 squamous cell carcinoma with local inflammation models that were established by inflammation was induced by inoculation VX2 tumor, mechanical trauma, and drinking of milk with high sugar viscosity. The animals were divided into four groups. Group A （n=12）： rabbit buccal VX2 squamous cell carcinoma with local inflammation, procaine penicillin was intramuscularly given, and tinidazole tablets were given by gavage for three consecutive days. Group B （n=12）： rabbit buccal VX2 squamous cell carcinoma with local inflammation, normal saline was intramuscularly given, and aspirin were given by gavage for three consecutive days. Group C （n=12）： rabbit buccal VX2 squamous cell carcinoma with local inflammation, normal saline was given intramuscularly and by gavage for three consecutive days. Group D （n=10）.＂ rabbit buccal VX2 squamous cell carcinoma, normal saline was given intramuscu- larly and by gavage for three consecutive days. All the rabbits were sacrificed for collection of tumor specimens, and the expression levels of membrane-surface HLA-DR and CD86 in DCs of tumor specimens were detected viaflow cytometry. Results The positive expression rate of HLA-DR and the double positive expression rate of HLA-DR and CD86 were group A〉 group D〉 group B〉 group C. The positive expression rate of CD86 were group A〉 group D〉 group B and group C （P〈 0.05）. Conclusion Anti-infection treatment significantly increased the expressions of HLA-DR and CD86 in DCs of rabbit buccal VX2 squamous cell carcinoma tissue complicated with local inflammation.