IL-10、IFN-γ对儿童噬血细胞综合征的检测意义

IL-10, IFN-γ for the study of the significance of detecting for children with hemophagocytic syndrome

目的:评价血清IL-6、IL-10、IL-18、IFN-γ对儿童噬血细胞综合征(HPS)的早期诊断及预后的价值。方法:采用前瞻性研究方法,对20例诊断为HPS患儿分成治疗前和治疗后,以20例正常体检儿童作为阴性正常对照组,再选取缓解期急性淋巴细胞白血病(ALL)18例和脓毒症患儿22例血清作为阳性对照组。通过流式细胞仪流式微球术对血清IL-6、IL-10、IL-18、IFN-γ水平进行测定。比较患儿治疗前后和不同分组之间血清4种细胞因子水平。结果:4种细胞因子水平随患儿HPS治疗前后明显变化,治疗前4种细胞因子水平明显高于治疗后和正常对照组,差异有统计学意义(P<0.05);HPS患儿IL-10、IFN-γ水平较正常对照组、ALL患儿组及脓毒症患儿组显著升高,差异有统计学意义(P<0.05)。结论:HPS患儿IL-10和IFN-γ水平相比ALL和脓毒症患儿显著升高,可以作为儿童HPS早期诊断和预测预后的临床指标。

Objective： To evaluate serum IL-6, IL-10, IL-18, IFN-γ for the value of early diagnosis and prognosis of children with hemophagocytic syndrome. Methods： By using the method of prospective study of 20 children with a diagnosis of HPS into diagnosis before treatment and after treatment, while at the same time, with 20 cases normal physical examination children as normal control group, then choose the remission of acute lymphoblastic leukemia（ALL）, 18 cases and sepsis were 22 cases of serum were chosen as positive control group. IL-6, IL-10, IL-18, IFN-γ levels were determined with flow cytometry instrument flow ball technique. Four kinds of cytokines serum levels before and after treatment between different groups were compared. Results： Four kinds of cytokines levels obriously varied with HPS before and after treatment in children, 4 kinds of cytokines during the diagnosis level was significantly higher than that of the normal control group after treatment（P〈0.05）, children with HPS of IL-10 and level of IFN-γ obviously elevated compared with normal controls, children with acute leukemia and the cases of sepsis was significant difference（P〈0.05）. Conclusion： Cytokines IL-10 and IFN-γ levels in children with HPS before treatment is significantly elevated compared with the control group, then markedly reduced for treatment, so it can be used as a children＇s early diagnosis and prognosis prediction of HPS clinical indicator.