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剪切力对间充质干细胞的细胞休眠状态的诱导作用

Laminar shear stress result in maintaining the quiescence of MSCs
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摘要 目的探讨层流剪切力(LSS)对间充质干细胞(MSCs)增殖和凋亡的影响。方法选择间充质干细胞(MSCs),并对其进行体外流体剪切力力学加载(生理范围内);通过DNA合成了解增殖情况,采用流式细胞仪及PCR技术检测其凋亡情况。结果在生理范围内的流体力学加载,细胞增殖与其加载力相关;该范围内的力学刺激对MSCs的凋亡起抑制作用。结论生理范围内的流体剪切力诱导MSCs进入休眠状态。 Objective To investigate the effects of laminar shear stress（ LSS） on the proliferation and apoptosis of mesenchymal stem cells（ MSCs）. Methods The influences of laminar shear stress（ the physiological levels） on MSCs were studied. DNA synthesis and cell cycle were measured,to detect the cellular proliferation. The apoptosis-related gene expression（ Bcl-2 / Bax） was examined to determine the effect of LSS. Results The percentage of MSCs＇ proliferation rate was reduced by the fluid shear stress. Furthermore,it was detected that LSS exerted a potent suppression effect on MSC apoptosis. Conclusion These data revealed a critical role of LSS in maintaining the quiescence of MSCs.
作者 罗伟 李锋 熊伟 高仕长
机构地区 重庆医科大学附属第一医院骨科 华中科技大学同济医学院附属同济医院骨科
出处 《局解手术学杂志》 2015年第2期123-126,共4页 Journal of Regional Anatomy and Operative Surgery
基金 国家自然基金面上项目(30772206)
关键词 间充质干细胞 剪切力 细胞周期抑制 凋亡 mesenchymal stem cells shear stress cell cycle arrest apoptosis
分类号 R329.25 [医药卫生—人体解剖和组织胚胎学]
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参考文献17

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