丹红对脑卒中后运动功能改善的实验研究

Laborotary study of Dan Hong in improving the movement function after stroke

目的观察丹红对脑梗塞再灌注大鼠运动功能改善的治疗作用。方法 SD大鼠制备大脑中动脉阻塞模型,再灌注24 h后随机分为对照组、丹红高剂量、丹红低剂量组及维脑路通组。对照组生理盐水腹腔注射,丹红高、低剂量组据大鼠体质量给予丹红注射液14.4 mg/kg和3.6 mg/kg腹腔注射,维脑路通组以0.04 g/kg腹腔注射给药。计算脑梗死灶面积,行横木行走实验,双前肢抓握力量检测。结果各组大鼠在24 h和3 d可见对照组梗塞面积最大,与其他各组比较,差异有统计学意义(P<0.05)。大鼠3 d时横木行走实验,对照组、维脑路通组、丹红高、低剂量组得分分别为:(1.30±0.91)分、(3.78±1.72)分、(4.18±2.05)分、(4.63±2.45)分,用药各组与对照组相比,有统计学差异(P<0.05)。药物组与对照组大鼠双前肢肌力有统计学差异(P<0.05)。结论丹红可明显提高大鼠运动功能恢复,且与维脑路通作用相当。

Objective To investigate Danhong in improving the movement function of reperfused rat after stroke. Methods Rats to make middle cerebral artery occlusion model,after 24 hours of reperfusion were divided into control group,high dose and low dose of Danhong groups and Venorruton group randomly. Rats in Control group were injected saline. High and low dose group were injected Dan Hong according to their weight,high dose as 14. 4 mg / kg,low dose as 3. 6 mg / kg. Venorruton group were injected Venorruton as 0. 04 g / kg. The infarcted square of rat brain was measured,the rats were tested with walking wood bar and two fore limb grasp strength. Results Rat infarcted squre in 24 hours and 3 days,control groups is the biggest,compared with others,there are statistically difference（ P〈0. 05）. Score in each group of walking wood bar in 3rd days are（1. 30 ± 0. 91）,（3. 78 ± 1. 72）,（ 4. 18 ± 2. 05）,（ 4. 63 ± 2. 45）. Compared between control and other druge groups,there are statistically differnec（ P〈0. 05）. There are statistically differences between control and other groups in two fore limb grasp testing（ P〈0. 05）. Conclusion Danhong has the same effect which can improve the movement function to stroke rat as Venorruton.