老年、高龄及长寿老人肝脾组织基因组DNA含量的变化及比较

Comparison of genomic DNA content of liver and spleen tissue for different age of elderly

目的观察尸检老年、高龄及长寿老人肝、脾组织基因组DNA含量的变化及差异,探讨衰老进程中DNA含量的变化规律并为今后的衰老相关研究提供实验数据。方法选取24 h内老年尸检（≥65岁）低温（-80℃）保存的肝组织35例和脾组织33例,按照年龄分为3组：65~79岁组、80~89岁组、≥90岁组（其中包括百岁老人2例）;低温（-80℃）保存的肝、脾组织用试剂盒提取基因组DNA,紫外光谱法测定肝脾组织DNA含量,并进行比较分析。结果肝组织DNA含量：与65~79岁组（1.464±0.488）mg/m L比较,80~89岁组（0.310±0.286）mg/m L显著降低（P〈0.05）;且≥90岁组（1.147±0.333）mg/m L的DNA含量显著高于80~89岁组（P〈0.05）。脾组织基因组DNA含量：与65~79岁组（1.723±0.726）mg/m L比较,80~89岁组（0.938±0.589）mg/m L显著降低（P〈0.05）;且≥90岁组（1.688±0.963）mg/m L的DNA含量显著高于80~89岁组（P〈0.05）。同一尸检者肝、脾组织DNA含量比较：肝组织平均DNA含量（0.856±0.658）mg/m L显著低于脾组织平均DNA含量（1.414±0.852）mg/m L（P〈0.05）。结论老年人肝、脾组织基因组DNA含量随增龄而降低;90岁以上肝、脾组织DNA的含量均有所增加;同一尸检者肝组织DNA含量显著低于脾组织DNA含量,提示不同脏器组织DNA含量存在差异。

Objective To investigate the change of genomic DNA of liver and spleen tissue for different age of the elderly,and provide the experimental data for aging-related research. Methods 35 livers and 33 spleens of autopsied samples preserved in refrigerator at- 80 ℃ were divided into 3 groups according to age：age 65 y to 79 y,age 80 y to 89 y,age≥90y. The content of DNA in liver and spleen was determined by ultraviolet absorbent method. Results Compaired with age 80 y to 89y（0. 310 ± 0. 286） mg / m L,the content of DNA in liver was significant higher at age 65 y to 79y（1. 464 ± 0. 488） mg / m L and age ≥ 90y（1. 147 ± 0. 333） mg / m L（ P〈0. 05）;Compared with age80 y to 89y（0. 938 ± 0. 589） mg / m L,the content of DNA in spleen was significant higher at age 65 y to 79y（1. 723 ± 0. 726） mg / m L and age≥90y（1. 688 ± 0. 963）mg/m L（P〈0. 05）. The content of DNA was significant lower in liver（0. 856 ± 0. 658）mg/m L than that in spleen（1. 414 ± 0. 852）mg/m L. Conclusion The content of DNA in human liver and spleen tissue may be decrease along with aging. The content of DNA in the group at age≥90y may be increase. There were some differences between different viscera tissue in content of DNA.