麦洼牦牛TF基因的克隆及蛋白质结构分析

Cloning and protein structure analysis of transferrin gene in Maiwa yak

[目的]探讨麦洼牦牛转铁蛋白(Transferrin,TF)的结构及生理功能。[方法]采用RT-PCR方法,从麦洼牦牛心脏克隆出TF基因,并运用多种生物信息学软件对其进行核苷酸序列及蛋白质结构分析。[结果]克隆得到的麦洼牦牛TF基因开放阅读框为780 bp,编码259个氨基酸,分子质量为28.33 k Da,理论等电点8.57,不含跨膜区域,存在信号肽序列,二级结构以随机卷曲和α-螺旋为主,无β-折叠,同源建模预测出牦牛TF蛋白与猪血清转铁蛋白有相似的三级结构,同源性可达71.91%。[结论]成功克隆了麦洼牦牛TF基因并分析了其蛋白质结构,为进一步研究TF蛋白功能提供理论基础。

[Objective]To explore the structure and physiological function of transferrin in Maiwa yak. [Methods]TF gene fragment was cloned from Maiwa Yak by RT-PCR,and protein structure analysis used a variety of bioinformatics softwares. [Results]Maiwa yak TF contained a 780 bp open reading frame,which encoded 259 amino acids. The molecular weight was 28. 33 k Da and theoretical IP was8. 57. The TF protein had no transmembrane regions,but had a signal peptide sequence. Its secondary structure was composed of random coil and α-helix,but no β-sheet. Homology modeling showed that TF protein of Maiwa yak had similar tertiary structure with porcine TF. [Conclusion]TF gene was obtained and protein structure was analyzed. It will benefit further study of TF function in Maiwa yak.