摘要
目的：研究调强照射模式对鼻咽癌CNE2细胞放射生物学效应的影响。方法克隆形成实验计算细胞存活分数，多靶单击模型拟合生存曲线，比较不同照射时间及剂量率对CNE2细胞放射生物学效应的影响。结果⑴调强模式10、15和20 min照射组与常规模式照射组比较，10 min照射组与常规模式组间放射生物学参数差异无统计学意义（ P ＞0．05）；余照射组间放射生物学参数[存活分数（SF）2、准阈剂量（Dq）、外推数（N）]差异均有统计学意义（ P ＜0．05）。⑵CNE2细胞接受出线率分别为300 Mu／min和600 Mu／min的照射后，SF2300 Mu／min＞SF2600 Mu／min，差异有统计学意义（ P ＜0．05）；余放射生物学参数间的差异均无统计学意义（ P ＞0．05）。结论⑴照射时间延长＜10 min可能对CNE2细胞的放射生物学相对效应影响不大，只有照射时间延长至＞15 min时，放射生物学效应才降低。⑵CNE2细胞的SF2随着该实验所用出线率的增加而降低，提高出线率可能弥补照射时间延长造成的剂量损失。
Objective To investigate the influence of intensity-modulated radiation pattern on ra-diobiological effects of nasopharyngeal carcinoma cell line CNE2.Methods Colony formation assay was used to calculate cell surviving fraction, and compare the cell survival curves and the surviving fraction with single-hit multi-target model fitting survival curves to different delivery time and dose rates.Results ⑴Nasopharyngeal carcinoma cell line CNE2 was irradiated on two models：conventional radiation （ CR） mod-el;intensity modulated radiation model （ IMRT model, which was divided into 10 min IMRT irradiated group, 15 min irradiated group, and 20 min irradiated group） .Except that the parameters of radiation biol-ogy did no significantly differ between CR model and 10 min IMRT irradiated group （ P 〉0.05） , the other groups among radiobiology parameters surviving fraction （SF2）, quasi-threshold dose（Dq） and extrapolation number（N） were all significant differences （ P 〈0.05） .⑵ They found that the SF2 300 Mu/min〉SF2 600 Mu/min （ P 〈0.05） when the CNE2 cells were treated with dose rates of 300 Mu /min and 600 Mu/min.Conclusions ⑴There were not significant changes in the biological effects until the delivery time prolonged to more than 10 minutes.When the delivery time extended to more than 15 minutes, the survival fraction was increasing with the radiation time risen.⑵The dose rates used in the experiment did not show any effect on the radiobiology parameters without SF2 .
出处
《中国医师杂志》
CAS
2015年第3期380-382,共3页
Journal of Chinese Physician
