纳米羟基磷灰石/胶原蛋白/丝素蛋白复合骨组织工程支架材料的生物相容性

Biocompatibility of nano-hydroxyapatite/collagen/silk fibroin composite scaffolds for bone tissue engineering

背景:通过将两种及两种以上材料共混制备复合支架材料可以弥补各自的不足,利用各种材料的互补特性来满足组织工程对支架的要求。目的:制备纳米羟基磷灰石/胶原蛋白/丝素蛋白复合三维支架材料,并研究其细胞相容性。方法:将纳米羟基磷灰石、胶原蛋白与丝素蛋白分别按质量比为1∶1∶5、1∶2∶5、1∶3∶5的比例混合,制备纳米羟基磷灰石/胶原蛋白/丝素蛋白复合材料,测试其孔隙率、孔径大小、吸水膨胀率及压缩力学性能。将表征结果良好的质量比为1∶2∶5的纳米羟基磷灰石/胶原蛋白/丝素蛋白复合材料与MC3T3-E1细胞体外复合培养,MTT法检测复合培养2,4,6,8,12 d后的细胞活性。结果与结论:羟基磷灰石/胶原蛋白/丝素蛋白按质量1∶2∶5的比例混合更符合要求:孔径98-260μm,孔隙率为(96.72±2.78)%,吸水膨胀率为(549.37±35.29)%,生物力学试验机测定其力学性能稳定、压缩应变及弹性模量等指标适宜骨组织工程研究应用。MC3T3-E1细胞在纳米羟基磷灰石/胶原蛋白/丝素蛋白复合三维支架上生长增殖良好,表明纳米羟基磷灰石/胶原/丝素复合三维支架具有良好的细胞相容性。

BACKGROUND：Composite scaffolds made of two or more materials can meet the requirements of tissue engineering scaffolds based on the complementary properties of various materials. OBJECTIVE： To prepare three-dimensional nano-hydroxyapatite （nHA）/colagen （COL）/silk fibroin （SF） scaffolds and to study their biocompatibility. METHODS： Composite scaffolds were prepared by mixing nHA, COL and SF at different ratios of 1：1：5, 1：2：5, 1：3：5. In order to find out the best mixing ratio of SF, COL and nHA, the porosity, pore size, water absorption and mechanical properties were determined. MC3T3-E1 cels were cultured in the nHA/COL/SF scaffolds composite scaffold （1：2：5）in vitro to observe the biocompatibility. Cel viability was detected after co-culture for 2, 4, 6, 8, 12 days using MTT. RESULTS AND CONCLUSION： It was more suitable for bone scaffolds when the mixed ratio of nHA, COL and SF was 1：2：5. The pore size was 98-260 μm, the porosity was （96.72±2.78）%, and the water absorption was （549.37±35.29）%. The stability of its mechanical properties, compressive strain and elastic modulus and other indicators were measured by biomechanical testing machine, which are suitable for bone tissue engineering applications. MC3T3-E1 cels grew wel in the nHA/COL/SF composite scaffold, indicating the composite scaffold has good cytocompatibility.