脑出血后大鼠神经干细胞增殖及迁徙途径的实验研究

The experimental study on proliferation and migration pathway of the neural stem cells in the rats following intracerebral hemorrhage

目的探讨脑出血后大鼠神经干细胞增殖变化及迁徙途径。方法将75只SD大鼠随机分为脑出血组、假手术组和正常组,脑出血组采用自体血注入法复制脑出血模型。各实验大鼠腹腔注射溴脱氧核苷尿嘧啶(Brd U)标记神经干细胞,运用免疫组化染色观察神经干细胞增殖及迁徙分布情况。结果脑出血组侧脑室下区室管膜上皮的Brd U阳性细胞数比假手术组和正常组显著增加(P<0.05),Brd U阳性细胞沿胼胝体迁徙,最后散在分布于出血灶及周边区。结论脑出血后神经干细胞可增殖并沿胼胝体向出血灶及周边区迁徙,参与出血性脑损伤神经功能的修复。

Objective To investigate proliferation and migration pathways of the neural stem cells in the rats following intracerebral hemorrhage（ICH）. Methods The 75 SD rats were randomly divided into the ICH group, sham group and normal group the ICH group received injection of autologous blood to establish the ICH model. The bromodeoxyuridine（Brd U） was used to mark neural stem cells. Proliferation,migration and distribution of neural stem cells were observed by immunohistochemical staining. Results Brd U-positive cells located in the ependymal epithelium of subventricular zone increased significantly in the ICH group compared with the control group and normal group（ P0.05）, and migrated along corpus callosum,and finally settled in the hemorrhagic focus and the surrounding area. Conclusion Neural stem cells in the rats following ICH can proliferate and migrate to the hemorrhagic focus and surrounding area along corpus callosum, and participate in repairing the nerve function of hemorrhagic brain injury.