去卵巢联合糖皮质激素诱发兔骨质疏松模型的研究

The research of ovariectomy combined with glucocorticoid for induction of osteoporosis in rabbits

目的建立去卵巢联合糖皮质激素致兔骨质疏松模型,探讨去卵巢联合糖皮质激素对兔骨代谢的影响。方法将18只新西兰雌兔随机分为3组：假手术组（SHAM,生理盐水5 ml·kg-1·d-1）;去卵巢组（OVX,生理盐水5 ml/kg/d）;去卵巢联合糖皮质激素组（OVX＋GC,地塞米松1 mg·kg-1·d-1）。实验第0、3和6周,检测各组大白兔腰椎（3-6）和右侧股骨的骨密度。实验结束时,取右侧股骨进行生物力学检测;取第3腰椎进行骨组织形态计量学检测。结果与SHAM组相比,OVX＋GC组大白兔骨密度、骨生物力学性能（最大载荷、断裂载荷等）、骨小梁面积百分数、骨小梁厚度和骨小梁数量均显著降低（P〈0.05）,骨小梁分离度显著升高（P〈0.05）,而OVX组大白兔的均无显著差异。结论去卵巢联合糖皮质激素法建立兔骨质疏松模型切实可行,而且效果优于单纯去卵巢兔骨质疏松模型。

Objective To explore the effect of ovariectomy combined with glucocorticoid on rabbits bone metabolism and develop a new method for inducing osteoporosis in rabbits. Method 18 New Zealand female rabbits were randomly divided into 3 groups： Sham-operated group（SHAM, Physiological saline 5 ml·kg- 1· d- 1）; ovariectomy group（OVX, Physiological saline 5 ml · kg- 1· d- 1）; ovariectomy combined with glucocorticoid group（OVX ＋GC, Dexamethasone 1 mg· kg- 1· d- 1）. The femoral bone mineral density（BMD）of the rabbit lumbar（3-6） and the right femoral were assessed at 0, 3 and 6-week, respectively. At the end of the experiment, the right femur biomechanics and the third lumbar vertebra bone tissue morphology metrology were determined. Result Compared with Sham- operated group, the BMD, the bone biomechanics（maximum load, fracture load）, the trabecular bone area percentage, the bone trabecular thickness and the bone trabecular number in the ovariectomy combined with glucocorticoid group were significantly decreased（P0.05）, the bone separating degree were significantly increased（P0.05）, while the ovariectomy group was not significantly different. Conclusion The method of ovariectomy combined with glucocorticoid is better at induction of osteoporosis in rabbits than ovariectomy alone and it is a feasible approach.