外源油菜素内酯(EBR)对Cu胁迫期间番茄幼苗的缓解效应

Alleviating Effects of Exogenous EBR on Tomato Seedlings During Copper Stress

采用营养液水培的方法,以‘改良毛粉802F1’番茄为供试材料,通过检测番茄植株内激素、根系分泌物、氨基酸、电解质渗漏率和生物量的变化,研究外源2,4-表油菜素内酯(2,4-EBR,EBR)对Cu胁迫下番茄的缓解效应及机制。结果表明,与Cu胁迫处理相比,喷施0.1 mg·L-1的EBR能显著提高叶片内源ZR和GA含量,显著降低根系中ZR、ABA和IAA的含量,同时提高番茄植株内各种有机酸的含量,其中柠檬酸含量升高482.2%,乳酸含量为0.187 mg·g-1。植株中氨基酸总量增加了11.1%,生物量增加20.9%。表明外源EBR可以降低Cu的生物毒性,减缓过多Cu对番茄幼苗的抑制作用。

Through nutrient solution cultivating, the tomato （Solanum lycopersicum） cultivar ＇Gailiang Maofen 802F1＇, was selected as the experiment material. The changes of hormones, root exudates, amino acids, electrolyte leakage rate and biomass were detected to study the alleviating effects and mechanism of 2,4-EBR on tomato seedlings under copper stress. The results showed that compared with copper stress treatment, spraying 0.1 mg·L^-1 EBR could significantly increase the contents of endogenous ZR and GA in leaves, while the ZR, ABA and IAA contents were remarkably reduced in roots. The contents of various organic acid in tomato plants were significantly improved at the same time, especially the citric acid was rised up to 482.2% and the content of the lactic acid was 0.187 mg·g^-1. The total amount of amino acid in tomato plants was increased 11.1% and the plant biomass was increased 20.9%. These results suggested exogenous EBR could reduce the biological toxicity of Cu, alleviate the inhibitory effect on tomato seedlings under superabundant Cu.