不同糖耐量人群血清吞噬细胞样还原型烟酰胺腺嘌呤二核苷酸磷酸氧化酶水平与胰岛β细胞功能的关系

Relationship between serum level of phagocyte-like nicotinamide adenine dinucleotide phosphate oxidase and pancreaticβ-cell function among different groups of glucose tolerance

目的：研究不同血糖水平下，体内总吞噬细胞样烟酰胺腺嘌呤二核苷酸氧化酶（Nox）含量与氧化应激水平，及其与胰岛β细胞功能的关系。方法根据空腹血糖（FBG）和口服糖耐量试验2 h血糖（OGTT 2 hPG），将84名体检市民分为正常糖耐量组（NGT组26例）：FPG〈6.1 mmol/L和OGTT 2 hPG〈7.8 mmol/L；糖调节受损组（IGR组，27例）：FPG在6.1-7.0 mmol/L之间，且OGTT 2 hPG〈7.8 mmol/L或FPG〈6.1 mmol/L，且OGTT 2 h PG在7.8-11.1 mmol/L之间或FPG在6.1-7.0 mmol/L之间，且OGTT 2 h PG 7.8-11.1 mmol/L之间；糖尿病组（DM组，31例）：FPG≥7.0 mmol/L和/或OGTT 2 hPG≥11.1 mmol/L。测定每组Nox含量、8-羟基脱氧鸟苷（8-OHdG）、超氧化物歧化酶（SOD）、丙二醛（MDA）水平。多组计量资料间比较采用方差分析，组间比较采用独立样本t检验，各因素与校正胰岛素抵抗后的胰岛β细胞功能指数（HBCI）行Pearson法相关分析和多元逐步回归分析。结果 DM组Nox水平较IGR组升高，HBCI较IGR组降低[分别为（143.3±22.1）比（118.0±21.8）U/L，12.4±2.3比31.1±7.7，t=2.156、5.621，均P〈0.05]；DM组Nox水平较NGT组明显升高，HBCI较NGT组明显降低[分别为（143.3±22.1）比（97.2±19.9）U/L，12.4±2.3比105.2±21.3，t=4.460、4.111，均P〈0.05]。Nox是HBCI的独立影响因素（r=-0.572，β=-1.088, R2=0.455，P〈0.001）。结论 Nox是反映机体氧化应激水平的一个较为理想指标，与胰岛β细胞功能之间存在密切联系。

Objective To evaluate the oxidative status under different blood glucose levels, and study the relationship between body phagocyte-like nicotinamide adenine dinucleotide phosphate oxidase （Nox） with oxidative stress and pancreaticβ-cell function. Methods According to the fasting blood-glucose and oral glucose tolerance test,84 individuals who participated physical exam were divided into 3 groups：normal glucose tolerance group（NGT,n=26, FPG〈6.1 mmol/L and OGTT 2 hPG〈7.8 mmol/L）, impaired glucose regulation group （IGR, n=27, FPG between 6.1-7.0 mmol/L and OGTT 2 hPG〈7.8 mmol/L）or FPG〈6.1 mmol/L and OGTT 2 h PG between 7.8-11.1 mmol/L,or FPG between 6.1-7.0 mmol/L and OGTT 2 h PG between 7.8-11.1 mmol/L, and diabetes mellitus group（n=31, FPG≥7.0 mmol/L ,and/or OGTT 2 hPG≥11.1 mmol/L）.Nox, 8-hydroxy-2′-deoxyguanosine （8-OHdG）, superoxide dismutase（SOD）, malondialdehyde（ MDA） was measured. The islet beta cell function index（HBCI）was calculated by each recognized formula. Variance analysis was used to analysis between multiple groups,and independent samples t testto comparetwo groups. Pearson′s correlation Multivariate regression analysis were performed between HBCI which was corrected by insulin resistance and other variable. Results With the increase of blood glucose, the level of Nox in DMgroup was higher than IGR group.HBCI was decreased（（143.3±22.1）vs（118.0±21.8）U/L, （12.4 ± 2.3）vs（31.1 ± 7.7）, t=2.156,5.621, respectively,all P〈0.05）. Nox in DM group was significantly higher than NGT,group. HBCI was significantly decreased（（143.3 ± 22.1）vs （97.2 ± 19.9）U/L,12.4 ± 2.3 vs105.2±21.3, t=4.460,4.111, respectively,all P〈0.05）. The further multiple regression analysis indicated that only Nox was independent factor on HBCI（r=-0.572,β=-1.088, R2=0.455,P〈0.001）. Conclusions Nox maybe an ideal index in reflecting the oxidative status in the body. Oxidative stress derived from Nox is closely tied to pancreaticβ-cell function.