潮汕地区虾蟹壳提取甲壳素的工艺优化

Optimization of Chitin Extraction from Shrimp and Crab Shell in Chaoshan Area

采用传统酸碱法提取潮汕地区虾蟹壳中甲壳素,对HCl溶液浓度、HCl溶液用量、酸浸时间3个因素进行正交试验,确定虾壳中脱钙的最佳工艺为提取5.00 g虾壳需HCl溶液浓度1.50 mol/L,酸浸时间6 h,HCl溶液用量50.00 m L,氢氧化钠溶液浓度为2.00%,碱浸时间24 h时,平氢氧化钠溶液用量36.30 m L,甲壳素含量为22.40%;蟹壳中脱钙的最佳工艺为5.00 g蟹壳需HCl溶液浓度1.50 mol/L,酸浸时间6 h,HCl溶液用量75.00 m L,氢氧化钠溶液浓度2.00%,碱浸时间24 h,平均氢氧化钠溶液用量25.03 m L,甲壳素含量为19.80%。

Using traditional acid-alkali method to extract chitin from the shrimp and crap shell in Chaoshan area, orthogonal experiment was conducted on the three elements of acid concentration, dosage and time to make sure the best extraction technology. The results showed that for decalcifying 5.00 g shrimp shell,the optimum extraction conditions were 1.50 mol / L HCl, 50.00 m L, treat 6 h, and the alkali concentration 2.00%, the average use of alkali 36.30 m L, treat 24 h, and then the yield of chitin was 22.40%. For crab shell, the best conditions were 1.50 mol / L HCl,75.00 m L and treat 6 h,and 2.00% alkali, 25.03 m L on average and treat 24 h, and then the yield of chitin was 19.80%.