RP-HPLC法同时测定藏药肋柱花中三种成分的含量

Content Analysis of Three Constituents in Tibetan Herb Lomatogonium rotatum(L.) Fries es Nym by RP-HPLC

建立了同时测定藏中当药黄素、当药醇苷、苦龙苷含量的方法。采用反相高效液相色谱法（RPHPLC）,以Kromasil-C18（250 mm×4.6 mm,5μm）为色谱柱,以甲醇（A）-0.04%磷酸溶液（B）为流动相,梯度洗脱,40%A（0 min）-63%A（20 min）;流速为0.8 m L/min;柱温为30℃,检测波长为254 nm。结果表明,在20 min内完成对3种成分的分析,各成分之间均达到基线分离。当药黄素、当药醇苷、苦龙苷的线性范围分别为：0.675 0~4.050 0μg（R2=0.999 5）、0.005 5~0.330 0μg（R2=0.999 5）、0.027 5~0.165 0μg（R2=0.999 5）,线性关系良好。平均加样回收率（n=9）分别为99.90%、99.15%、99.88%。建立的HPLC分析方法简单、准确,能快速测定藏药肋柱花中有效成分的含量。

RP-HPLC was used to determine the content of swertisin, swertianolin, amarogerth in Tibetan herb. The sample was separated on the column of Kromasil-C18（250 mm×4.6 mm,5 μm） eluted with methanol（A） and 0.04% phosphoric acid solution（B）. The initial condition was 40%A, then rose to 63% A in 20 min with detection wavelength at 254 nm, flow rate0.8 m L / min, and column temperature 30 ℃. And three active constituents were base-isolated. The linear ranges of swertisin,swertianolin, amarogerth, were 0.675 0~4.050 0 μg（R2=0.999 5）, 0.005 5~0.330 0 μg（R2=0.999 5）, and 0.027 5~0.165 0 μg（R2=0.999 5）respectively; the average recoveries（n=9） were 99.90%, 99.15% and 99.88% respectively. The method was easy and precise which can be used for content analysis of active constituents in Lomatogonium rotatum（L.）Fries es Nym rapidly.