QIAcube小型工作站在生物接触类检材提取中的应用研究

QIAcube System in Touch DNA Extraction

目的探讨QIAcube工作站在生物接触类检材中的提取效果。方法将90份以脱落细胞粘取膜、实物样本(纱线转移)或棉签擦拭物为载体的生物接触类检材,分别采用QIAcube工作站、EZ1工作站和手工硅珠法进行基因组DNA提取、定量并检测15个常染色体基因座的STR分型。结果利用QIAcube工作站提取的生物接触类检材平均检出率达到44.4%,其中脱落细胞粘取膜组检出率为56.7%,实物纱线转移组检出率为46.7%,棉签擦拭物组检出率为30.0%。脱落细胞粘取膜组和实物样本(纱线转移)组均获得较好的STR分型结果,所得DNA浓度均优于棉签擦拭物组样本。QIAcube工作站组平均检出率为44.4%,手工硅珠法组平均检出率为43.2%,而EZ1工作站组平均检出率为26.9%,前两者平均检出率明显高于EZ1工作站组。QIAcube工作站组与硅珠法组所得DNA浓度无显著差异,但均明显高于EZ1工作站组。QIAcube工作站组与硅珠法组检测得到的STR分型RFU值及峰值均衡性均优于EZ1工作站组,尤其是大片段基因在前两组中均能够被完整检测到。结论采用QIAcube工作站提取生物接触类检材检出率高,STR分型结果良好,可应用于法医物证实际案件检验。

Objective To explore the applicability of QIAcube Forensics Casework DNA extraction system into extracting touch DNA and evaluate the effect. Methods 30 samples were transferred from forensic biological evidence using tape-lifting technique, 30 samples transferred by yarns and 30 samples transferred using double swab technique. All these 90 samples were extracted by QIAcube, EZ1 and Si O2 extraction systems respectively, then quantified and detected by STR typing. Results Up to 44.4% touch DNA were successfully detected by QIAcube extraction system, with successful rate of 56.7% in tape-lifting group, 46.7% in yarns group and 30.0% in double swab group. Both forensic biological evidence of tape lifting group and yarns group got good STR typing results using QIAcube extraction system,and the DNA concentrations were higher than that of swab group. QIAcube and Si O2 extraction system got good STR typing, especially at the large fragment loci,and the concentrations of extracted DNA were higher than that of EZ1 extraction system. Up to 44.4% touch DNA were detected by QIAcube extraction system, while the Si O2 extraction system was 43.2% and the EZ1 extraction system was 26.9%. There was no statistical difference in DNA concentration between QIAcube and Si O2 system, which were both obviously higher than that of EZ1 extraction system. Conclusions QIAcube extraction system is an effective method to extract DNA from forensic biological evidence, applicable into forensic practice.