云南部分鼠疫疫源地中小肠结肠炎耶尔森菌的分离及鉴定

Isolation and identification of Yersinia enterocolitica in selected plague foci in Yunnan

目的了解云南部分鼠疫疫源地中小肠结肠炎耶尔森菌的分布及病原学特征。方法 2012年5月至2014年4月采集剑川、梁河两县鼠类盲肠,对其进行培养,通过菌落聚合酶链反应初筛小肠结肠炎耶尔森菌,通过生化鉴定确定菌株并作毒力基因检测。结果 915份标本中分离到小肠结肠炎耶尔森菌35株,总检出率为3.83%,分离株包含1株致病株、34株非致病株。35株小肠结肠炎耶尔森菌全部为生物1A型,1株致病株为1A/O∶9生物血清型,毒力基因为(ail+、yst A-、yst B-、yad A-、vir F-、rbfc-),非致病株血清为O∶5、O∶8及未分型,毒力基因为(ail-、yst A-、yst B+、yad A-、vir F-、rbfc-)。结论小肠结肠炎耶尔森菌在剑川野鼠鼠疫疫源地鼠间流行,分离菌株血清型别较为多样,分离到的致病株缺乏典型毒力基因;小肠结肠炎耶尔森菌在梁河家鼠鼠疫疫源地尚未发现鼠间的流行。

Objective To understand distribution of Yersinia enterocolitica and their etiology in selected plague foci in Yunnan province. Methods The mouse＇s cecum were collected in Jianchuan and Lianghe counties, Y. enterocolitica were detected by using colony PCR, the strains＇ biochemical identification and virulence genes detection were conducted. Results A total of 35 strains of Y. enterocolitica were isolated from 915 specimens （ 3.83% ）, only 1 strain was pathogenic. All the 35 strains belonged to biological type 1A. The pathogenic strain belonged to type 1A/O： 9, and it＇s virulence genes included ail - , ystA- , ystB- , yadA- , virF- , rbfc-. The non-pathogenic strains belonged to serotype 1A/O： 5, O： 8 and were unspecified, and their virulence genes included ail-, ystA-, ystB＋ , yadA-, virF-, rbfc-. Condusion Y. enterocolitica circulated in plague focus in Jianchuan, the serotypes of the strains varied and no typical virulence gene was detected in the pathogenic strain isolated. No Y. enterocolitica strain was detected in plague focus in Lianghe.